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Purified Rat Anti-Mouse F4/80
Purified Rat Anti-Mouse F4/80
Analysis of Mouse F4/80 Expression      Left and Middle Panel: Immunohistochemical analysis of Mouse F4/80 expression in mouse spleen and small intestine. Acetone-fixed, frozen C57BL/6 mouse splenic (Left Panel) and small intestinal (Middle Panel) sections were stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927; Left Images) or Purified Rat Anti-Mouse F4/80 antibody (Cat. No. 565409; Right Images) as indicated in each panel. A three-step staining procedure that employed Biotin Goat Anti-Rat Immunoglobulin (Cat. No. 559286), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification 20x.      Right Panel: Flow cytometric analysis of F4/80 on Mouse splenic monocytes. Right Panel: C57BL/6 mouse splenic leucocytes were stained with either Purified Rat IgG2a, κ Isotype Control (dashed line histogram) or Purified Rat Anti-Mouse F4/80 antibody (solid line histogram). After washing, the cells were secondarily stained with PE Goat Anti-Rat Ig (Cat. No. 550767). The fluorescence histogram showing F4/80 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Analysis of Mouse F4/80 Expression      Left and Middle Panel: Immunohistochemical analysis of Mouse F4/80 expression in mouse spleen and small intestine. Acetone-fixed, frozen C57BL/6 mouse splenic (Left Panel) and small intestinal (Middle Panel) sections were stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927; Left Images) or Purified Rat Anti-Mouse F4/80 antibody (Cat. No. 565409; Right Images) as indicated in each panel. A three-step staining procedure that employed Biotin Goat Anti-Rat Immunoglobulin (Cat. No. 559286), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification 20x.      Right Panel: Flow cytometric analysis of F4/80 on Mouse splenic monocytes. Right Panel: C57BL/6 mouse splenic leucocytes were stained with either Purified Rat IgG2a, κ Isotype Control (dashed line histogram) or Purified Rat Anti-Mouse F4/80 antibody (solid line histogram). After washing, the cells were secondarily stained with PE Goat Anti-Rat Ig (Cat. No. 550767). The fluorescence histogram showing F4/80 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
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BD Pharmingen™
Gpf480; F480; Emr1; Ly71; DD7A5-7; EGF-TM7; TM7LN3
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse F4/80 Recombinant Protein
Flow cytometry (Routinely Tested), Immunohistochemistry (Tested During Development)
0.5 mg/ml
AB_2739222
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  5. An isotype control should be used at the same concentration as the antibody of interest.
565409 Rev. 1
Antibody Details
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T45-2342

The T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). F4/80 is a 160 kDa glycoprotein that belongs to the EGF-TM7 family of seven-transmembrane spanning cell surface molecules. It is expressed on the surface of granulocytes and a wide range of mature tissue macrophages including, Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria macrophages, and Langerhans cells. F4/80 expression has also been reported on subpopulations of dendritic cells. F4/80 expression is heterogeneous and may be increased during inflammatory responses as observed in various mouse models of colitis, diabetes and brain injury.

        

565409 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
565409 Rev.1
Citations & References
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Development References (5)

  1. Austyn JM., and Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981; 10:805-815. (Biology). View Reference
  2. Gordon S, Hamann J, Lin HH, Stacey M. F4/80 and the related adhesion-GPCRs. Eur J Immunol. 2011; 41(9):2472-2476. (Biology). View Reference
  3. Krüger T, Benke D, Eitner F, et al. Identification and functional characterization of dendritic cells in the healthy murine kidney and in experimental glomerulonephritis. J Am Soc Nephrol. 2004; 15(3):613-621. (Biology). View Reference
  4. Leenen PJ, Radosević K, Voerman JS, et al. Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover.. J Immunol. 1998; 160(5):2166-73. (Biology). View Reference
  5. McKnight AJ, Macfarlane AJ, Dri P, Turley L, Willis AC, Gordon S. Molecular cloning of F4/80, a murine macrophage-restricted cell surface glycoprotein with Homology to the G-protein-linked transmembrane & hormone receptor family. J Biol Chem. 1996; 271:486. (Biology). View Reference
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565409 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.