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    BV421 Mouse Anti-Human CD68
    BV421 Mouse Anti-Human CD68
    Flow cytometric analysis of CD68 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either BD Horizon™ BV421 Mouse IgG2b, κ Isotype Control (Cat. No. 562748; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD68 antibody (Cat. No. 564943; solid line histogram).  The fluorescence histogram showing CD68 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    BV421 Mouse Anti-Human CD68
    Flow cytometric analysis of CD68 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either BD Horizon™ BV421 Mouse IgG2b, κ Isotype Control (Cat. No. 562748; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD68 antibody (Cat. No. 564943; solid line histogram).  The fluorescence histogram showing CD68 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    Product Details
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    BD Horizon™
    GP110; Macrosialin; SCARD1; Scavenger receptor class D, member 1
    Human (QC Testing)
    Mouse BALB/c IgG2b, κ
    PHA-stimulated Human PBMC
    Intracellular staining (flow cytometry) (Routinely Tested)
    5 µl
    IV M149; VI MR23
    AB_2739020
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. An isotype control should be used at the same concentration as the antibody of interest.
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    564943 Rev. 2
    Antibody Details
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    Y1/82A

    The Y1/82A monoclonal antibody specifically binds to CD68 which is also known as, Scavenger receptor class D member 1 (SCARD1), Macrosialin, or GP110. CD68 is a cell surface 110 kDa-type I-transmembrane glycoprotein that is primarily expressed in cytoplasmic granules of monocytes, macrophages, dendritic cells, granulocytes, myeloid progenitor cells and, reportedly, a subset of CD34-positive hemopoietic bone marrow progenitor cells. CD68 belongs to the sialomucin family and serves as a scavenger receptor that can bind and internalize oxidized low density lipoproteins (LDL). This antibody is useful in studies of myeloid cell development and function.

    The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

    564943 Rev. 2
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    564943 Rev.2
    Citations & References
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    Development References (5)

    1. Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988; 41(7):753-758. (Immunogen: Flow cytometry, Immunohistochemistry). View Reference
    2. Davey FR, Erber WN, Gatter KC, Mason DY. The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias. Am J Clin Pathol. 1988; 89(1):76-80. (Clone-specific: Immunohistochemistry). View Reference
    3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
    4. Stockinger H. Cluster report: CD68. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:841-843.
    5. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
    View All (5) View Less
    564943 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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