Two-color flow cytometric analysis of human CRACC (CD319) expression on peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody (Cat. No. 555333/561808/561809) and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 558053; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human CRACC (CD319) antibody (Cat. No. 564338; Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots show the correlated expression patterns of CRACC (CD319) [or Ig Isotype control staining] versus CD3 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Two-color flow cytometric analysis of human CRACC (CD319) expression on peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody (Cat. No. 555333/561808/561809) and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 558053; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human CRACC (CD319) antibody (Cat. No. 564338; Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots show the correlated expression patterns of CRACC (CD319) [or Ig Isotype control staining] versus CD3 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
CRACC; SLAMF7; 19A; 19A24 protein; CS1
Human (QC Testing)
Mouse IgG2a, κ
Human CRACC/SLAMF7
Flow cytometry (Routinely Tested)
5 µl/test
AB_2738754
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
50 Tests
Cat No.
558053
PE Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
555333
PE Mouse Anti-Human CD3 RUO
Size
25 Tests
Cat No.
561808
564338 Rev. 1
Antibody Details
235614
The 235614 monoclonal antibody specifically binds to CRACC (CD2-like receptor activating cytotoxic cells) which is also known as CD319, CS1 (CD2 subset 1), SLAMF7, 19A, and 19A24. CRACC is a type I transmembrane glycoprotein that belongs to the CD2 family of receptors within the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8+ T cells, CD4+ T cells, B cells, macrophages, and dendritic cells. CRACC reportedly plays roles in the activation, proliferation and effector functions of T cells, NK cells, and other leucocytes.
564338 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564338 Rev.1
Citations & References
Development References (8)
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Boles KS, Mathew PA. Molecular cloning of CS1, a novel human natural killer cell receptor belonging to the CD2 subset of the immunoglobulin superfamily. Immunogenetics. 2001; 52(3-4):302-307. (Biology). View Reference
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Bouchon A, Cella M, Grierson HL, Cohen JI, Colonna M. Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family. J Immunol. 2001; 167(10):5517-5521. (Biology). View Reference
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Fasth AE, Bjorkstrom NK, Anthoni M, Malmberg KJ, Malmstrom V. Activating NK-cell receptors co-stimulate CD4(+)CD28(-) T cells in patients with rheumatoid arthritis. Eur J Immunol. 2010; 40(2):378-387. (Biology). View Reference
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Hagberg N, Theorell J, Schlums H, Eloranta ML, Bryceson YT, Ronnblom L. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. J Immunol. 2013; 191(6):2989-2998. (Biology). View Reference
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Kim JR, Horton NC, Mathew SO, Mathew PA. CS1 (SLAMF7) inhibits production of proinflammatory cytokines by activated monocytes. Inflamm Res. 2013; 62(8):765-772. (Biology). View Reference
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Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol Lett. 2011; 134(2):113-121. (Biology). View Reference
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Murphy JJ, Hobby P, Vilarino-Varela J, et al. A novel immunoglobulin superfamily receptor (19A) related to CD2 is expressed on activated lymphocytes and promotes homotypic B-cell adhesion. Biochem J. 2002; 361(Pt3):431-436. (Biology). View Reference
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Tassi I, Colonna M. The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells. J Immunol. 2005; 175(12):7996-8002. (Biology). View Reference
564338 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
