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PE Mouse anti-Mouse IL-23 Receptor
PE Mouse anti-Mouse IL-23 Receptor
Flow cytometric analysis of mouse IL-23 Receptor (IL-23R) expression on IL-23R-non-transfected and IL-23R transfected cells. Mouse IL-23R-non-transfected (Left Panel) and IL-23R-transfected (Right Panel) cells were stained with either PE Mouse IgG1, κ Isotype Control  (Cat. No. 554680, dashed line histogram) or PE Mouse anti-Mouse IL-23 Receptor (Cat. No. 562468; solid line histogram). Flow cytometric fluorescence histograms showing the expression of IL-23R (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of mouse IL-23 Receptor (IL-23R) expression on IL-23R-non-transfected and IL-23R transfected cells. Mouse IL-23R-non-transfected (Left Panel) and IL-23R-transfected (Right Panel) cells were stained with either PE Mouse IgG1, κ Isotype Control  (Cat. No. 554680, dashed line histogram) or PE Mouse anti-Mouse IL-23 Receptor (Cat. No. 562468; solid line histogram). Flow cytometric fluorescence histograms showing the expression of IL-23R (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
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BD Pharmingen™
Il23r; IL-23R; Interleukin 23 receptor
Mouse (QC Testing)
Mouse IgG1, κ
Mouse IL-23 Receptor Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
209590
AB_11154593
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562468 Rev. 2
Antibody Details
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3C9

The 3C9 monoclonal antibody specifically binds to the mouse Interleukin-23 Receptor (IL-23R) subunit that is encoded by the il23r gene. The IL-23R subunit is a type I transmembrane glycoprotein and member of the hemopoietin receptor superfamily. The mouse IL-23 Receptor complex is comprised of IL-23R and IL-12 receptor beta 1 (IL-12Rβ1) subunits. The IL-23R complex can bind IL-23, a cytokine that plays roles in innate and adaptive immunity as well as in autoimmune diseases, eg, by the generation and maintenance of Th17 cells. Mouse IL-23R is expressed by activated/memory CD4+ T cells, Th1, Th2 and Th17 cells, γδ T cells, dendritic cells and macrophages as determined by IL-23R mRNA expression and IL-23R-GFP reporter mouse studies. The IL-23-bound IL-23R complex transduces an intracellular signal pathway mediated by a Jak-STAT signaling cascade.

562468 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
562468 Rev.2
Citations & References
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Development References (6)

  1. Awasthi A, Riol-Blanco L, Jager A, et al. Cutting edge: IL-23 receptor gfp reporter mice reveal distinct populations of IL-17-producing cells. J Immunol. 2009; 182(10):5904-5908. (Biology). View Reference
  2. Jones LL, Alli R, Li B, Geiger TL. Differential T Cell Cytokine Receptivity and Not Signal Quality Distinguishes IL-6 and IL-10 Signaling during Th17 Differentiation.. J Immunol. 2016; 196(7):2973-85. (Biology). View Reference
  3. Lankford CS, Frucht DM. A unique role for IL-23 in promoting cellular immunity. J Leukoc Biol. 2003; 73(1):49-56. (Biology). View Reference
  4. Monk JM, Hou TY, Turk HF, McMurray DN, Chapkin RS. n3 PUFAs reduce mouse CD4+ T-cell ex vivo polarization into Th17 cells.. J Nutr. 2013; 143(9):1501-8. (Biology). View Reference
  5. Parham C, Chirica M, Timans J, et al. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002; 168(11):5699-5708. (Biology). View Reference
  6. Petermann F, Rothhammer V, Claussen MC, et al. gammadelta T cells enhance autoimmunity by restraining regulatory T cell responses via an interleukin-23-dependent mechanism. Immunity. 2010; 33(3):351-363. (Biology). View Reference
View All (6) View Less
562468 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.