Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Middle East / Africa (English)
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
    Alert Icon
    PE-CF594 Mouse Anti-Human CD90
    PE-CF594 Mouse Anti-Human CD90
    Flow cytometric analysis of CD90 expression on human HEL cell line. HEL cells (Human erythroleukemia cell line, ATCC, TIB-180™) were stained with BD Horizon™ PE-CF594 Mouse Anti-Human CD90 antibody (Cat. No. 562385, solid line histogram) or a BD Horizon™ PE-CF594 mIgG1, κ Isotype Control (Cat. No. 562292; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.
    PE-CF594 Mouse Anti-Human CD90
    Flow cytometric analysis of CD90 expression on human HEL cell line. HEL cells (Human erythroleukemia cell line, ATCC, TIB-180™) were stained with BD Horizon™ PE-CF594 Mouse Anti-Human CD90 antibody (Cat. No. 562385, solid line histogram) or a BD Horizon™ PE-CF594 mIgG1, κ Isotype Control (Cat. No. 562292; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.
    Product Details
    Down Arrow Up Arrow


    BD Horizon™
    THY1; Thy-1 antigen; Thy-1 membrane glycoprotein
    Human (QC Testing), Rhesus, Cynomolgus, Baboon, Pig, Dog (Tested in Development)
    Mouse BALB/c IgG1, κ
    Human HEL Cell Line
    Flow cytometry (Routinely Tested)
    5 µl
    V M07, BP222; VI BP28, E046
    7070
    AB_11154395
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
    Show More blue down arrow Show Less blue up arrow

    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. CF™ is a trademark of Biotium, Inc.
    8. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
    9. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
    10. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
    11. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    12. All other brands are trademarks of their respective owners.
    13. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
    14. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    Show More blue down arrow Show Less blue up arrow
    562385 Rev. 2
    Antibody Details
    Down Arrow Up Arrow
    5E10

    The 5E10 monoclonal antibody specifically binds to human CD90 which is also known as Thy-1. CD90 is a 25-35 kDa glycophosphatidylinositol-anchored membrane glycoprotein of the Ig superfamily that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. The anti-CD90 antibody binds to a subset of immature CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is highly enriched for cells capable of long-term culture. The anti-CD90 antibody is useful for enriching high proliferative potential colony-forming cells (HIPP-CFC) that are primative progenitor cells.

    This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).      

    562385 Rev. 2
    Format Details
    Down Arrow Up Arrow
    PE-CF594
    BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    PE-CF594
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    615 nm
    562385 Rev.2
    Citations & References
    Down Arrow Up Arrow

    Development References (6)

    1. Baum CM, Weissman IL, Tsukamoto AS, Buckle AM, Peault B. Isolation of a candidate human hematopoietic stem-cell population. Proc Natl Acad Sci U S A. 1992; 89(7):2804-2808. (Biology). View Reference
    2. Craig W, Kay R, Cutler RL, Lansdorp PM. Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med. 1993; 177(5):1331-1342. (Biology). View Reference
    3. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). View Reference
    4. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
    5. Lansdorp PM, Thomas TE. AP Gee, ed. Bone Marrow Processing and Purging. Boca Raton FL: CRC Press; 1991.
    6. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
    View All (6) View Less
    562385 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

    Website Feedback