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    V450 Rat Anti-Mouse IgG1
    V450 Rat Anti-Mouse IgG1
    Flow cytometric analysis of BD Horizon™ V450 Rat anti-Mouse IgG1 second step. C57BL/6 mouse splenocytes were first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The cells were washed and then stained with either Purified Mouse Anti-Mouse CD22.2 antibody (clone Cy34.1, solid line histogram) or with no antibody (dashed line histogram). After washing the cells were stained with BD Horizon™ V450 Rat Anti-Mouse IgG1 (Cat. No. 562107) as the second step. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
    V450 Rat Anti-Mouse IgG1
    Flow cytometric analysis of BD Horizon™ V450 Rat anti-Mouse IgG1 second step. C57BL/6 mouse splenocytes were first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The cells were washed and then stained with either Purified Mouse Anti-Mouse CD22.2 antibody (clone Cy34.1, solid line histogram) or with no antibody (dashed line histogram). After washing the cells were stained with BD Horizon™ V450 Rat Anti-Mouse IgG1 (Cat. No. 562107) as the second step. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
    Product Details
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    BD Horizon™
    Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
    Pooled Mouse IgG1
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    16017
    AB_10894002
    Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
    6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
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    562107 Rev. 1
    Antibody Details
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    A85-1

    The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

    The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

    562107 Rev. 1
    Format Details
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    V450
    BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    V450
    Violet 405 nm
    405 nm
    450 nm
    562107 Rev.1
    Citations & References
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    Development References (1)

    1. Honjo T, Obata M, Yamawaki-Katoaka Y, et al. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene. Cell. 1979; 18(2):559-568. (Biology). View Reference
    562107 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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