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    Alexa Fluor® 647 Mouse Anti-Human C3a Receptor
    Alexa Fluor® 647 Mouse Anti-Human C3a Receptor
    Flow cytometric analysis of C3a Receptor (C3aR) on human peripheral blood leukocytes. Human whole blood was stained with Alexa Fluor® 647 Mouse Anti-Human C3a Receptor (Cat. No. 561497; solid line histogram) or with an Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 557903; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel) or granulocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.
    Alexa Fluor® 647 Mouse Anti-Human C3a Receptor
    Flow cytometric analysis of C3a Receptor (C3aR) on human peripheral blood leukocytes. Human whole blood was stained with Alexa Fluor® 647 Mouse Anti-Human C3a Receptor (Cat. No. 561497; solid line histogram) or with an Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 557903; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel) or granulocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.
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    BD Pharmingen™
    C3AR; C3a-R; C3AR1; C3a anaphylatoxin chemotactic receptor; AZ3B
    Human (QC Testing)
    Mouse BALB/c IgG2b
    Human C3aR Transfected Cell Line
    Flow cytometry (Routinely Tested)
    5 µl
    AB_10715833
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
    5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
    7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    561497 Rev. 2
    Antibody Details
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    hC3aRZ8

    The hC3aRZ8 monoclonal antibody specifically binds to the human C3a Receptor (C3aR). C3aR is a seven-transmembrane glycoprotein, G-protein-coupled receptor that is the specific receptor for C3a anaphylatoxin. The C3aR consists of 482 amino acids forming a single polypeptide chain that is encoded by the C3AR1 gene located on chromosome 12 (location 12p13.31). C3aR are expressed by eosinophils, basophils, neutrophils, dendritic cells, monocytes, macrophages,  endothelial cells and some T cells. C3a is a bioactive cleavage product released from Complement Component 3 (C3) during complement activation. C3a plays a role in a variety of cellular immune responses as well as being a potent pro-inflammatory agent.  In response to bound C3a, this receptor stimulates cellular responses including chemotaxis, granule enzyme release and superoxide anion production and causes increased vascular permeability. In vivo, C3a production can initiate, contribute to, or exacerbate the inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ARDS, ischemic heart disease, post-dialysis syndrome, and several autoimmune diseases including rheumatoid arthritis, lupus erythematosus, and acute glomerulonephritis.

    561497 Rev. 2
    Format Details
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    Alexa Fluor™ 647
    Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    Alexa Fluor™ 647
    Red 627-640 nm
    653 nm
    669 nm
    561497 Rev.2
    Citations & References
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    Development References (8)

    1. Ames RS, Li Y, Sarau HM. Molecular cloning and characterization of the human anaphylatoxin C3a receptor. J Biol Chem. 1996; 271(34):20231-20234. (Biology). View Reference
    2. Crass T, Raffetseder U, Martin U. Expression cloning of the human C3a anaphylatoxin receptor (C3aR) from differentiated U-937 cells. Eur J Immunol. 1996; 26(8):1944-1950. (Biology). View Reference
    3. Ember JA, Hugli TE. C3a Receptor. In: Oppenheim JJ, Feldmann M, Durum SK. editors in chief, Joost J. Oppenheim, Marc Feldman ; editors, Scott K. Durum .. et al., ed. Cytokine reference : a compendium of cytokines and other mediators of host defense. London: Academic Press; 2001:2173-2181.
    4. Sacks SH. Complement fragments C3a and C5a: The salt and pepper of the immune response. Eur J Immunol. 2010; 40(3):668-670. (Biology). View Reference
    5. Soruri A, Kiafard Z, Dettmer C, Riggert J, Köhl J, Zwirner J. IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo.. J Immunol. 2003; 170(6):3306-14. (Immunogen). View Reference
    6. Strainic MG, Liu J, Huang D, et al. Locally produced complement fragments C5a and C3a provide both costimulatory and survival signals to naive CD4+ T cells.. Immunity. 2008; 28(3):425-35. (Biology). View Reference
    7. Werfel T, Kirchhoff K, Wittmann M, et al. Activated human T lymphocytes express a functional C3a receptor. J Immunol. 2000; 165(11):6599-6605. (Biology). View Reference
    8. Zwirner J, Gotze O, Begemann G, Kapp A, Kirchhoff K, Werfel T. Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies. Immunology. 1999; 97(1):166-172. (Biology). View Reference
    View All (8) View Less
    561497 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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