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    V450 Mouse Anti-Human CD73
    V450 Mouse Anti-Human CD73
    Flow cytometric analysis of CD73 expression on human mesenchymal stem cells. Human mesenchymal stem cells (Lonza) at passage 5 were harvested using BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527) and stained with BD Horizon™ V450 Mouse anti-Human CD73 antibody (Cat. No. 561255; solid line histogram) or a BD Horizon™ V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometry System.
    V450 Mouse Anti-Human CD73
    Flow cytometric analysis of CD73 on human lymphocytes. Whole blood was stained with BD Horizon™ V450 Mouse anti-Human CD73 (Cat. No. 561255; solid line fluorescence histogram) and compared with whole blood stained with BD Horizon™ V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373; used at a matching concentration; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II flow cytometry system.
    V450 Mouse Anti-Human CD73 V450 Mouse Anti-Human CD73
    Flow cytometric analysis of CD73 expression on human mesenchymal stem cells. Human mesenchymal stem cells (Lonza) at passage 5 were harvested using BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527) and stained with BD Horizon™ V450 Mouse anti-Human CD73 antibody (Cat. No. 561255; solid line histogram) or a BD Horizon™ V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometry System.
    Flow cytometric analysis of CD73 on human lymphocytes. Whole blood was stained with BD Horizon™ V450 Mouse anti-Human CD73 (Cat. No. 561255; solid line fluorescence histogram) and compared with whole blood stained with BD Horizon™ V450 Mouse IgG1, κ Isotype Control (Cat. No. 560373; used at a matching concentration; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II flow cytometry system.
    Product Details
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    BD Horizon™
    NT5E; 5' nucleotidase; 5'-NT; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5
    Human (QC Testing)
    Mouse IgG1, κ
    Pre-B leukemia cell line
    Flow cytometry (Routinely Tested)
    5 µl
    V B-CD73.3
    4907
    AB_11151899
    Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.
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    Product Notices

    1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    4. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
    5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    6. Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
    7. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    8. An isotype control should be used at the same concentration as the antibody of interest.
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    561255 Rev. 1
    Antibody Details
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    AD2

    The AD2 monoclonal antibody specifically binds to ecto-5'-nucleotidase, a 70 kDa, glycosyl phosphatidylinositol (GPI)-anchored glycoprotein. CD73 is expressed on bone marrow derived multipotent mesenchymal stem cells (MSCs), also sometimes identified as mesenchymal stem cells, and is one of the three positive markers that has been identified by the International Society for Cell Terapy (ISCT) for the minimum criteria for identifying MSCs.  Additionally CD73 is expressed on subsets of T and B lymphocytes, follicular dendritic cells, epithelial cells, and endothelial cells. Its expression on lymphocytes increases during T and B cell development. CD73 has enzymatic activity and catalyzes the dephosphorylation of adenosine monophosphate (AMP) converting it to adenosine. It has been suggested that CD73 can mediate costimulatory signals in T cell activation and adhesion of lymphocytes to endothelium.  Due to the expression characteristics of this molecule on different cell types, this BD Horizon™ V450 format is only recommended for use on cells expressing high amounts of CD73, such as MSCs.

    The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

    561255 Rev. 1
    Format Details
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    V450
    BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    V450
    Violet 405 nm
    405 nm
    450 nm
    561255 Rev.1
    Citations & References
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    Development References (6)

    1. Airas L, Salmi M, Jalkanen S. Lymphocyte-vascular adhesion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium. J Immunol. 1993; 151(8):4228-4238. (Biology). View Reference
    2. Alam MS, Kurtz CC, Rowlett RM, et al. CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice. J Infect Dis. 2009; 199(4):494-504. (Biology). View Reference
    3. Dominici M, Le Blanc K, Mueller I, et. al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-317. (Biology). View Reference
    4. Salazar-Gonzalez JF, Moody DJ, Giorgi JV, Martinez-Maza O, Mitsuyasu RT, Fahey JL. Reduced ecto-5'-nucleotidase activity and enhanced OKT10 and HLA-DR expression on CD8 (T suppressor/cytotoxic) lymphocytes in the acquired immune deficiency syndrome: evidence of CD8 cell immaturity. J Immunol. 1985; 135(3):1778-1785. (Biology). View Reference
    5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
    6. Thomson LF, Ruedi JM, Glass A, et al. Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). Tissue Antigens. 1990; 35(1):9-19. (Clone-specific). View Reference
    View All (6) View Less
    561255 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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