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    FITC Mouse anti-Human CD144
    FITC Mouse anti-Human CD144
    Analysis of CD144 on Human Umbilical Vein Endothelial Cells (HUVECs). HUVECs were stained with either FITC Mouse anti-Human CD144 (solid line) or FITC Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 555748, dotted line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
    FITC Mouse anti-Human CD144
    Analysis of CD144 on Human Umbilical Vein Endothelial Cells (HUVECs). HUVECs were stained with either FITC Mouse anti-Human CD144 (solid line) or FITC Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 555748, dotted line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
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    BD Pharmingen™
    VE-cadherin; Cadherin-5; CDH5; Vascular endothelial cadherin
    Human (QC Testing)
    Mouse IgG1, κ
    Flow cytometry (Routinely Tested)
    20 µl
    VI E092
    1003
    AB_1645487
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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    Antibody Details
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    55-7H1

    The 55-7H1 antibody monoclonal antibody specifically binds to a calcium-independent epitope on Cadherin-5, a member of the cadherin family of calcium-dependent adhesion molecules. Cadherin-5 is also known as CD144 or VE-Cadherin. CD144 is expressed on endothelial cells in vivo and in vitro. It may play a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium.

    Format Details
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    FITC
    Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    FITC
    Blue 488 nm
    494 nm
    518 nm
    Citations & References
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    Development References (3)

    1. Breier G, Breviario F, Caveda L, et al. Molecular cloning and expression of murine vascular endothelial-cadherin in early stage development of cardiovascular system.. Blood. 1996; 87(2):630-41. (Biology). View Reference
    2. Lampugnani MG, Resnati M, Raiteri M, et al. A novel endothelial-specific membrane protein is a marker of cell-cell contacts.. J Cell Biol. 1992; 118(6):1511-22. (Biology). View Reference
    3. Vincent PA, Xiao K, Buckley KM, Kowalczyk AP. VE-cadherin: adhesion at arm's length.. Am J Physiol, Cell Physiol. 2004; 286(5):C987-97. (Biology). View Reference

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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