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    APC Rat Anti-Mouse Vα2 TCR
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    This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
    APC Rat Anti-Mouse Vα2 TCR
    Flow cytometric analysis of Vα2 TcR on mouse lymph node cells.  Lymph node cells from BALB/c mice were stained with FITC Rat Anti-Mouse CD4 (Cat. No. 553046) and FITC Rat Anti-Mouse CD8b.2 (Cat. No. 553040) in addition to either a APC Rat IgG2a, λ isotype control (left panel) or with the APC Rat Anti-Mouse Vα2 TcR antibody (right panel).  Dot plots were derived from gated events based on light scattering characteristics for lymph node cells.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.
    APC Rat Anti-Mouse Vα2 TCR
    Flow cytometric analysis of Vα2 TcR on mouse lymph node cells.  Lymph node cells from BALB/c mice were stained with FITC Rat Anti-Mouse CD4 (Cat. No. 553046) and FITC Rat Anti-Mouse CD8b.2 (Cat. No. 553040) in addition to either a APC Rat IgG2a, λ isotype control (left panel) or with the APC Rat Anti-Mouse Vα2 TcR antibody (right panel).  Dot plots were derived from gated events based on light scattering characteristics for lymph node cells.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.
    Product Details
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    BD Pharmingen™
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
    Soluble αβ TCR from mouse cytotoxic T-cell clone KB5-C20
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    111660
    AB_1727582
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    560622 Rev. 1
    Antibody Details
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    B20.1

    The B20.1 monoclonal antibody specifically binds to most members of the Vα2 T-cell Receptor (TCR) subfamily in mice having the a, b, and c haplotypes of the Tcrb gene complex. B20.1 antibody may crossreact with Vδ8 TCR, which shares >90% sequence homology with Vα2 TCR. Levels of B20.1+ T cells appear to be influenced by Vα haplotypes. Moreover, the frequencies of Vα2+ CD8+ and CD4+ T cells are influenced by H-2 haplotypes.

    560622 Rev. 1
    Format Details
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    APC
    Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    APC
    Red 627-640 nm
    651 nm
    660 nm
    560622 Rev.1
    Citations & References
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    Development References (3)

    1. Grégoire C, Rebaï N, Schweisguth F, et al. Engineered secreted T-cell receptor alpha beta heterodimers.. Proc Natl Acad Sci USA. 1991; 88(18):8077-81. (Biology). View Reference
    2. Pircher H, Rebaï N, Groettrup M, et al. Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody.. Eur J Immunol. 1992; 22(2):399-404. (Immunogen). View Reference
    3. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
    560622 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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