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    PE Mouse Anti-Human CD122
    PE Mouse Anti-Human CD122
    Flow cytometric analysis of CD122 expression on human peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human CD122 (Cat. No. 554525; solid line histogram) or PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
    PE Mouse Anti-Human CD122
    Flow cytometric analysis of CD122 expression on human peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human CD122 (Cat. No. 554525; solid line histogram) or PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
    Product Details
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    BD Pharmingen™
    IL2RB; IL-2Rβ; IL-2R subunit beta; IL-2RB; IL15RB; IL-2/15Rb; IL-2R p75
    Human (QC Testing)
    Mouse BALB/c IgG1, κ
    Human YTS Cell Line
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    V C046
    3560
    AB_395453
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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    Recommended Assay Procedures

    This antibody has also been reported to be useful for immunoprecipitation experiments. It has been reported to precipitate from YTS cell lysates a 90,000 kD complex (IL-2 + p75). This antibody has also been reported not to block IL-2 binding to the β chain of the IL-2 receptor. Please note that this application is not routinely tested at BD Biosciences.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    554525 Rev. 2
    Antibody Details
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    Mik-β3

    The Mik-β3 monoclonal antibody specifically binds to CD122. CD122 is also known as IL-2/15Rβ because it is the shared p75 subunit (IL-12Rβ/IL-15Rβ) of heteromeric receptors for IL-2 and IL-15. CD122 is a 70-75 kD type I transmembrane glycoprotein expressed on  thymocytes, T cells, B cells, NK cells, monocytes, hematopoietic progenitor cells, fetal liver cells and stromal cells. The CD122 plays roles in the activation, differentiation and proliferation of various cell types including T cells, B cells and NK cells.

    554525 Rev. 2
    Format Details
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    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    554525 Rev.2
    Citations & References
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    Development References (3)

    1. Ishikawa T, Uchiyama T, Kamio M, Onishi R, Kodaka T, Okuma M. IL-4 down-regulates IL-2 receptor p75 by accelerating its endocytosis. Int Immunol. 1991; 3(6):517-525. (Biology). View Reference
    2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
    3. Tsudo M, Kitamura F, Miyasaka M. Characterization of the interleukin 2 receptor beta chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. 1989; 86(6):1982-1986. (Biology). View Reference
    554525 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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