Skip to main content Skip to navigation
PE Mouse Anti-Human CD275
Alert icon
This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [753603] or for additional support, contact your local applications specialist. Contact Us #
PE Mouse Anti-Human CD275
Flow cytometric analysis of CD275 expression on human CHO-B7-H2 cell line. CHO-B7-H2 cells were stained with either PE Mouse Anti-Human CD275 (Cat. No. 552502; solid line histogram) or PE Mouse IgG2b κ Isotype Control (Cat. No. 555743; dashed line histogram). Fluorescent histograms were derived from gated events with the forward and light-scattering characteristics of viable cells.
Flow cytometric analysis of CD275 expression on human CHO-B7-H2 cell line. CHO-B7-H2 cells were stained with either PE Mouse Anti-Human CD275 (Cat. No. 552502; solid line histogram) or PE Mouse IgG2b κ Isotype Control (Cat. No. 555743; dashed line histogram). Fluorescent histograms were derived from gated events with the forward and light-scattering characteristics of viable cells.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
ICOSLG; ICOS ligand; LICOS; B7-H2; B7 homolog 2; B7RP-1; GL50
Human (QC Testing)
Mouse IgG2b, κ
Human B7-H2
Flow cytometry (Routinely Tested)
20 µl
VIII 80191; IX 239
23308
AB_394404
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
552502 Rev. 5
Antibody Details
Down Arrow Up Arrow
2D3/B7-H2

The 2D3 monoclonal antibody specifically binds to CD275, which is also known as B7 homolog 2 (B7-H2/B7H2) and B7-related protein 1 (B7RP-1). CD275 is a type I transmembrane glycoprotein that belongs to the Ig superfamily. CD275 is likewise known as Inducible T-cell co-stimulator ligand (ICOS Ligand /ICOSLG/ICOS-L). It can bind to CD278 (ICOS) and costimulate the proliferation and cytokine production of antigen-stimulated human T cells. CD275 is variable expressed by subsets of B cells, monocytes, macrophages, dendritic cells and endothelial cells.

552502 Rev. 5
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
552502 Rev.5
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Bretscher PA. A two-step, two-signal model for the primary activation of precursor helper T cells. Proc Natl Acad Sci U S A. 1999; 96(1):185-190. (Biology). View Reference
  2. Dong H, Zhu G, Tamada K, Chen L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999; 5(12):1365-1369. (Biology). View Reference
  3. Wang S, Zhu G, Chapoval AI, et al. Costimulation of T cells by B7-H2, a B7-like molecule that binds ICOS. Blood. 2000; 96(8):2808-2813. (Biology). View Reference
552502 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.