FITC Mouse Anti-Rat CD5

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BD Pharmingen™ FITC Mouse Anti-Rat CD5

Name: FITC Mouse Anti-Rat CD5
Brand: BD Pharmingen™
SKU: 551449

Clone OX-19

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Rat (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Rat Thymocyte Lentil Lectin-binding Glycoproteins

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_394199

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

FITC Mouse IgG1, κ Isotype Control RUO

Size 0.25 mg Cat No. 550616

551449 Rev. 9

Antibody Details

OX-19

The OX-19 antibody reacts with CD5, a 69 kDa cell-surface glycoprotein found on thymocytes, peripheral T lymphocytes, and some thymic dendritic cells, but not on alloantigen-activated cytotoxic T cells, NK cells, γδ TCR-bearing intestinal intraepithelial lymphocytes, or dendritic epidermal T cells. A CD5+ subset of B lymphocytes has not been characterized in the rat. In the mouse and human, CD72 is the major ligand for CD5. While the OX-19 antibody is not mitogenic, its presence augments in vitro proliferative responses of T cells to lectins and allogeneic cells.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

551449 Rev. 9

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

551449 Rev.9

Citations & References

Development References (8)

Bañuls MP, Alvarez A, Ferrero I, Zapata A, Ardavin C. Cell-surface marker analysis of rat thymic dendritic cells. Immunology. 1993; 79(2):298-304. (Clone-specific). View Reference
Bellgrau D, Talmage DW. T cells can be cytotoxic without making interleukin 2: a model of separate pathways of induction. Proc Natl Acad Sci U S A. 1986; 83(10):3412-3416. (Biology). View Reference
Dallman MJ, Thomas ML, Green JR. MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur J Immunol. 1984; 14(3):260-267. (Immunogen). View Reference
Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). View Reference
Kuhnlein P, Park JH, Herrmann T, Elbe A, Hunig T. Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994; 153(3):979-986. (Biology). View Reference
Lawetzky A, Tiefenthaler G, Kubo R, Hunig T. Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta. Eur J Immunol. 1990; 20(2):343-349. (Biology). View Reference
Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K. Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72). J Immunol. 1992; 148(6):1630-1634. (Biology). View Reference
Vermeer LA, de Boer NK, Bucci C, Bos NA, Kroese FG, Alberti S. MRC OX19 recognizes the rat CD5 surface glycoprotein, but does not provide evidence for a population of CD5bright B cells. Eur J Immunol. 1994; 24(3):585-592. (Clone-specific). View Reference

View All (8)

551449 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.