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    Biotin Rat Anti-Mouse IgG1
    Product Details
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    BD Pharmingen™
    Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
    Pooled Mouse IgG1
    ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
    500 µg/ml
    16017
    AB_2296342
    Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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    Recommended Assay Procedures

    For immunohistochemical staining of acetone-fixed frozen sections, a dilution range of 1:50 - 1:200 is suggested.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
    5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    550331 Rev. 3
    Antibody Details
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    A85-1

    The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

    550331 Rev. 3
    Format Details
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    Biotin
    Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
    Biotin
    550331 Rev.3

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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