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Cytometer Setup & Tracking Beads Kit (use with BD FACSDiva™ software v 6.x) RUO

Cytometer Setup & Tracking Beads Kit (use with BD FACSDiva™ software v 6.x) 

  • Brand BD™
  • Storage Buffer Phosphate buffered saline with BSA and sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

BD™ Cytometer Setup and Tracking beads are for research use only with BD FACSDiva™ software (v 6.x). The beads allow the software to automatically characterize, track, and report measurements of supported BD digital flow cytometers. Each vial of beads contains equal concentrations of beads of three fluorescence emission intensities: bright, mid, and dim. The beads are used to define a baseline and run daily measurements of the cytometer. Each 3-mL vial contains beads sufficient for 50 daily measurements or 16 baseline definitions. Cytometer Setup and Tracking beads are for research use only with BD FACSDiva™ software (v 6.x). The beads allow the software to automatically characterize, track, and report measurements of supported BD digital flow cytometers.

Resources & Tools
 Spectrum Viewer  Data Sheet  Download TDS  Regulatory Document Website
Preparation and Storage

• Store the beads vial at 2°C to 8°C and protect from light. • Do not freeze Cytometer Setup and Tracking beads. • Vial contents are stable for the period shown on the vial label when stored as directed. Do not use after the expiration date. • Cytometer Setup and Tracking beads can be diluted in BD FACSFlow™ solution, BD FACSFlow solution with surfactant, or PBS. (See Procedure.) For consistent results, BD recommends always using the same diluent and sample delivery device to run Cytometer Setup and Tracking beads • After dilution, the beads suspension is stable for 8 hours at 2°C to 25°C when protected from light.


  1. Chase ES, Hoffman RA. Resolution of dimly fluorescent particles: a practical measure of fluorescence sensitivity. Cytometry. 1998;33:267-279.

  2. Establishing optimum baseline PMT gains to maximize resolution on BD Biosciences digital flow cytometers. BD application note. 23-8359-00. 2005.

  3. Hoffman RA. Standardization and quantitation in flow cytometry. In: Darzynkiewicz Z, Crissman HA, Robinson JP, eds. Methods in Cell Biology. Elsevier Inc; 2001;63:300-340.

  4. Hoffman RA. Standardization, calibration, and control in flow cytometry. Revised. In: Robinson JP, et al, eds. Current Protocols in Cytometry. New York, NY: John Wiley and Sons, Inc; 2005. Unit 1.3.

  5. Steen HB. Noise, sensitivity, and resolution of flow cytometers. Cytometry. 1992;13:822-830.

  6. Wood JC, Hoffman RA. Evaluating fluorescence sensitivity on flow cytometers: an overview. Cytometry. 1998;33:256-259.

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