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Purified Mouse Anti-ROR2
Purified Mouse Anti-ROR2
Western blot and flow cytometric analyses of ROR2 expression in human chronic myelogenous leukemia cell line. LEFT: Lysate prepared from K562 cells (ATCC CCL-243) was electrophoresed (SDS-PAGE), transferred to PVDF membranes and probed with 500, 250, 125 and 62.5 ng/mL (lanes 1-4, respectively) of Purified Mouse Anti-ROR2 (Cat. No. 565550). HRP Goat Anti-Mouse Ig (Cat. No. 554002) was used to develop the specific staining. Human ROR2 was identified as a band whose size ranged from 100-150 kDa. RIGHT: K562 cells (ATCC CCL-243) were fixed in BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized in BD Perm/Wash™ buffer (Cat. No. 554723). Permeablized cells were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse Anti-ROR2 (Cat. No. 565550, solid line), followed by PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Cell Analyzer.
Purified Mouse Anti-ROR2
Immunohistochemical staining of ROR2 in human and rat tissue sections. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; left column) or Purified Mouse Anti-ROR2 antibody (Cat. No. 565550; right column). A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Top Row: Human cerebellum. Original magnification: 10× Middle Row: Rat cerebellum. Original magnification: 20× Bottom Row: Rat embryo. Original magnification: 20×
Western blot and flow cytometric analyses of ROR2 expression in human chronic myelogenous leukemia cell line. LEFT: Lysate prepared from K562 cells (ATCC CCL-243) was electrophoresed (SDS-PAGE), transferred to PVDF membranes and probed with 500, 250, 125 and 62.5 ng/mL (lanes 1-4, respectively) of Purified Mouse Anti-ROR2 (Cat. No. 565550). HRP Goat Anti-Mouse Ig (Cat. No. 554002) was used to develop the specific staining. Human ROR2 was identified as a band whose size ranged from 100-150 kDa. RIGHT: K562 cells (ATCC CCL-243) were fixed in BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized in BD Perm/Wash™ buffer (Cat. No. 554723). Permeablized cells were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse Anti-ROR2 (Cat. No. 565550, solid line), followed by PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Cell Analyzer.
Immunohistochemical staining of ROR2 in human and rat tissue sections. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; left column) or Purified Mouse Anti-ROR2 antibody (Cat. No. 565550; right column). A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Top Row: Human cerebellum. Original magnification: 10× Middle Row: Rat cerebellum. Original magnification: 20× Bottom Row: Rat embryo. Original magnification: 20×
Product Details
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BD Pharmingen™
Neurotrophic tyrosine kinase, receptor-related 2; NTRKR2, BDB, BDB1
Human (QC Testing), Rat (Tested in Development), Mouse (Reported)
Mouse IgG1, κ
Mouse ROR2 Peptide
Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Intracellular staining (flow cytometry) (Tested During Development)
~105 kDa
0.5 mg/ml
AB_2739291
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
565550 Rev. 1
Antibody Details
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Ror2

The Ror2 monoclonal antibody specifically binds to the ST2 intracellular domain of ROR2 (Receptor tyrosine kinase-like Orphan Receptor 2). ROR2 is a type I transmembrane protein that belongs to a small family of receptor tyrosine kinases containing frizzled domains. Based on the homology of ROR2 and Wnt receptors Frz domains, ROR2 has been linked to Wnt signaling and embryonic development. Wnt5a-ROR2 signaling promotes osteoclastogenesis, and ROR2 is also critical for the formation of skeleton, heart, and genitals. In normal adult tissues, ROR2 is expressed in very low levels or undetectable. There is mounting evidence that ROR2 expression is associated with either tumor progression or suppression in different tissues. In humans, mutations in the ROR2 gene are associated with the autosomal recessive form of  Robinow Syndrome and autosomal dominant Brachydactyly type B.

565550 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
565550 Rev.1
Citations & References
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Development References (3)

  1. Ford CE, Qian Ma SS, Quadir A, Ward RL. The dual role of the novel Wnt receptor tyrosine kinase, ROR2, in human carcinogenesis. Int J Cancer. 2013; 133(4):779-787. (Biology). View Reference
  2. Mikels A, Minami Y, Nusse R. Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling. J Biol Chem. 2009; 284(44):30167-30176. (Immunogen: Immunohistochemistry, Western blot). View Reference
  3. van Amerongen R, Fuerer C, Mizutani M, Nusse R. Wnt5a can both activate and repress Wnt/β-catenin signaling during mouse embryonic development. Dev Biol. 2012; 369(1):101-114. (Clone-specific: Immunohistochemistry, Western blot). View Reference
565550 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.