Purified NA/LE Rat Anti-Human IL-4
Clone MP4-25D2 (RUO)
- Brand BD Pharmingen™
- Concentration 1.0 mg/ml
- Isotype Rat IgG1
- Reactivity Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
Neutralization (Tested During Development)
- Immunogen Purified Recombinant Human IL-4
- Storage Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
- Regulatory Status RUO
Regulatory Status Legend
The MP4-25D2 monoclonal antibody specifically binds to Interleukin-4 (IL-4). IL-4 is also known as Lymphocyte stimulatory factor 1, B cell stimulatory factor 1 (BSF-1), or B cell growth factor 1 (BCGF-1). IL-4 is produced by activated T cells, basophils, and mast cells. IL-4 is a multifunctional cytokine and growth factor that affects a variety of different target cell types. IL-4 can costimulate T cell proliferation and survival, as well as help drive Th2-like cell differentiation and effector functions. It costimulates B cell proliferation and survival, and can help B cells differentiate into IgG4- or IgE-producing cells. IL-4 can also diminish the proinflammatory functions of monocytes and macrophages. IL-4 exerts its biological effects by binding with high affinity to cell surface CD124 (IL-4Rα chain). CD124 forms signaling IL-4 receptor complexes with either CD132/common γ chain or CD213a1/IL-13Rα1 to form either Type I or Type II IL-4 Receptor complexes, respectively. The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to crossreact with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described.
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Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
ELISA: Biotin Rat Anti-Human IL-4 antibody (Clone MP4-25D2, Cat. No. 554483) has been reported to be useful as a detection antibody for sandwich ELISA measuring human IL-4 protein levels. Biotinylated MP4-25D2 antibody can be paired with the Purified Mouse Anti-Human IL-4 antibody (Clone 8D4-8, Cat. No. 554515/556917) as the capture antibody, with recombinant human IL-4 (Cat. No. 554605) as the standard. For measuring human IL-4 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IL-4 ELISA Set (Cat. No. 555194) or BD OptEIA™ Human IL-4 ELISA Kit II (Cat. No. 550614).