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    APC Mouse Anti-Rat CD3
    APC Mouse Anti-Rat CD3
    Two-color analysis of the expression of CD3 on rat splenocytes. A single-cell suspension of Lewis splenocytes was simultaneously incubated with the APC-conjugated 1F4 mAb and FITC-conjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
    APC Mouse Anti-Rat CD3
    Two-color analysis of the expression of CD3 on rat splenocytes. A single-cell suspension of Lewis splenocytes was simultaneously incubated with the APC-conjugated 1F4 mAb and FITC-conjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
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    BD Pharmingen™
    Rat (QC Testing)
    Mouse BALB/c IgM, κ
    F344 rat splenic T cells stimulated with PMA plus A23187
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_398622
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Recommended Assay Procedures

    The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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    557030 Rev. 5
    Antibody Details
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    1F4

    The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

    557030 Rev. 5
    Format Details
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    APC
    Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    APC
    Red 627-640 nm
    651 nm
    660 nm
    557030 Rev.5
    Citations & References
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    Development References (3)

    1. Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). View Reference
    2. Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Biology). View Reference
    3. Tanaka T, Masuko T, Yagita H, Tamura T, Hashimoto Y. Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody.. J Immunol. 1989; 142(8):2791-2795. (Immunogen). View Reference
    557030 Rev. 5

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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