Biotin Mouse Anti-Human CD196 (CCR6)
Clone 11A9 (RUO)
- Brand BD Pharmingen™
- Alternative Name BN-1; C-C CKR-6; C-C chemokine receptor type 6; CC-CKR-6; CCR-6
- Concentration 0.5 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing) Rhesus, Cynomolgus, Baboon (Tested in Development)
- Application
Flow cytometry (Routinely Tested)
- Immunogen Human CD196/CCR6 Peptide
- Workshop No. IX 48
- Entrez Gene ID 1235
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Description
The 11A9 monoclonal antibody specifically binds to CD196, which is also known as CCR6. CCR6 is a seven-transmembrane, G-protein-coupled, glycoprotein receptor that is a member of the beta chemokine receptor family. The human CCR6 gene has been mapped to chromosome 6q27. CCR6 is a receptor for the CC chemokine CCL20/MIP-3alpha/LARC/Exodus and also binds with lower affinity to and mediates responses to beta-defensin2/hBD-2. CCR6 is predominantly expressed by B lymphocytes, certain subsets of effector and memory T cells and by immature dendritic cells but not by monocytes, NK cells, or granulocytes. Skin-homing CLA (Cutaneous Lymphocyte Antigen)-positive memory T cells, Th1 cells, regulatory T cells and IL-17A-producing Th17 cells predominantly express high levels of CCR6. CCR6 mediates the trafficking of T, B, and dendritic cells to epithelial sites near the skin and mucosal surfaces during inflammatory and immunological responses. An N-terminal peptide of human CCR6 was used as an immunogen to generate the 11A9 hybridoma. The 11A9 antibody does not cross-react with human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CCR8, CCR9, CXCR1, CXCR2, CXCR3, CXCR4 and CXCR5 receptors. This antibody is NOT a neutralizing antibody.
Format
Suggested Companion Products
Biotin Mouse IgG1 κ Isotype Control RUO
100 Tests
Cat No: 555747
PE Streptavidin RUO
0.5 mg
Cat No: 554061
Stain Buffer (FBS) RUO
500 mL
Cat No: 554656
Stain Buffer (BSA) RUO
500 mL
Cat No: 554657
Lysing Solution 10X Concentrate CE/IVD
100
Cat No: 349202
Lysing Buffer RUO
100 mL
Cat No: 555899
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The biotinylated 11A9 antibody (Cat. No. 559561) can be used for the immunofluorescent staining and flow cytometric analyses of human leukocytes (see image) and cell lines that express CCR6. A titration range of 0.1-1.0 µg/test is recommended for biotinylated 11A9. The data in the figure represents staining of human peripheral lymphocytes with 0.25 µg biotinylated 11A9 and ≤ 0.06 µg/test of streptavidin-phycoerythrin (Cat. No. 554061).