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Purified Mouse Anti-Stat1 (pY701)
Purified Mouse Anti-Stat1 (pY701)
A431 cells were either left untreated (left lane) or treated (right lane) with 100 ng/ml EGF for 5 minutes at 37°C. The top panel was probed with Stat1 (Cat. No. 610115) and the bottom was probed with Stat1 (pY701) (Cat. No. 612232).
Purified Mouse Anti-Stat1 (pY701)
A431 cells were serum starved and treated with EGF (100 ng/ml) for 5 minutes, then fixed in 3.75% paraformaldehyde with 0.2% Triton X-100. Immunofluorescent staining was performed with Stat1 (Cat. No. 610115) and Stat1 (pY701) (Cat. No. 612232).
A431 cells were either left untreated (left lane) or treated (right lane) with 100 ng/ml EGF for 5 minutes at 37°C. The top panel was probed with Stat1 (Cat. No. 610115) and the bottom was probed with Stat1 (pY701) (Cat. No. 612232).
A431 cells were serum starved and treated with EGF (100 ng/ml) for 5 minutes, then fixed in 3.75% paraformaldehyde with 0.2% Triton X-100. Immunofluorescent staining was performed with Stat1 (Cat. No. 610115) and Stat1 (pY701) (Cat. No. 612232).
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG2a
Phosphorylated Human Stat1 Peptide
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development)
84/91 kDa
250 µg/ml
AB_399555
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612232 Rev. 1
Antibody Details
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4a

Stat (Signal transducer and activators of transcription) proteins mediate the biological activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors.  Ligand-receptor interaction activates constitutively-associated JAK family kinases as well as subsequent recruitment and activation of Stat proteins by tyrosine phosphorylation.  Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes.  Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner.  Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, the primary transcription activator induced by interferon binding to a specific cell-surface receptor.  Stat1 has two alternatively spliced isoforms, 91-kDa Stat1α and 84-kDa Stat1β; Stat1α has 38 additional C-terminal amino acids.  In response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex translocates to the nucleus. This forms an active complex that includes the DNA-binding p48 subunit, and is responsible for modulating interferon-stimulated genes (ISGs) transciption.

The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1α and Stat1β.

612232 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612232 Rev.1
Citations & References
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Development References (2)

  1. Darnell JE Jr. STATs and gene regulation. Science. 1997; 277(5332):1630-1635. (Biology). View Reference
  2. Fu XY, Zhang JJ. Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter. Cell. 1993; 74(6):1135-1145. (Biology). View Reference
612232 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.