Purified Mouse Anti-Human CD62L
Clone SK11 (RUO)
- Brand BD Pharmingen™
- Alternative Name L-selectin; LECAM-1; SELL; LNHR; LSEL; LAM-1; LEU8; PLNHR; TQ-1; MEL-14
- Concentration 1.0 mg/ml
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
Western blot, Functional assay (Tested During Development)
- Immunogen Human Peripheral Blood T Lymphocytes
- Workshop No. V S059
- Entrez Gene ID 6402
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The SK11 monoclonal antibody specifically binds to CD62L which is also known as, L-selectin, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1/LECAM-1), Leukocyte adhesion molecule 1 (LAM1/LAM-1), Lymph node homing receptor (LNHR), Leu-8, or TQ1. CD62L is an ~80 kDa type I transmembrane glycoprotein that belongs to the Selectin/LECAM family. CD62L is differentially expressed on T cells, B cells, monocytes, granulocytes, and a subset of NK cells. The CD62L molecule is the human homolog of the mouse lymph node homing receptor, MEL-14. CD62L plays a role in leucocyte binding to inflamed endothelium and extravasation, as well as mediating lymphocyte homing into peripheral lymphoid tissues through high endothelial postcapillary venules. Soluble CD62L can result from the proteolytic cleavage of cell surface CD62L during cellular activation or inflammation.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Functional Studies: Anti-LECAM-1 monoclonal antibodies have been shown to inhibit neutrophil localization at sites of acute inflammation. Anti-LECAM-1 monoclonal antibody (clone SK11) also induces suppressor function in CD4+ T lymphocytes but does not induce lymphocyte proliferation.
Immunoblotting: Using anti-LECAM-1 monoclonal antibody (clone SK11) in Western blot analysis of extracts from Leu- 8-transfected COS cells and the human T-cell leukemia line (Jurkat) revealed at least seven species of similar molecular weight and at least two closely spaced bands of 76 kD, the largest molecular weight form seen in COS cells.