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    APC Mouse Anti-Human CD137
    APC Mouse Anti-Human CD137
    Profile of Con A-stimulated peripheral blood mononuclear cells (PBMC) analyzed by flow cytometry.
    APC Mouse Anti-Human CD137
    Flow cytometric analysis of CD137 expression on stimulated human peripheral blood lymphocytes. Concanavalin A (Con-A)-stimulated (3 days) peripheral blood mononuclear cells were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram) or APC Mouse Anti-Human CD137 antibody (Cat. No. 550890/561702; solid line histogram). The fluorescence histogram showing the expressed levels of CD137 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
    APC Mouse Anti-Human CD137 APC Mouse Anti-Human CD137
    Profile of Con A-stimulated peripheral blood mononuclear cells (PBMC) analyzed by flow cytometry.
    Flow cytometric analysis of CD137 expression on stimulated human peripheral blood lymphocytes. Concanavalin A (Con-A)-stimulated (3 days) peripheral blood mononuclear cells were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram) or APC Mouse Anti-Human CD137 antibody (Cat. No. 550890/561702; solid line histogram). The fluorescence histogram showing the expressed levels of CD137 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
    Product Details
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    BD Pharmingen™
    4-1BB, ILA, TNFRSF9
    Human (QC Testing)
    Mouse BALB/c IgG1, κ
    Recombinant Human 4-1BB Fusion Protein
    Flow cytometry (Routinely Tested)
    20 µl
    VI C-7
    3604
    AB_398477
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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    Recommended Assay Procedures

    BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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    561702 Rev. 2
    Antibody Details
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    4B4-1

    The 4B4-1 monoclonal antibody specifically binds to CD137 which is also known as 4-1BB, and ILA (induced by lymphocyte activation). CD137 is a type I transmembrane glycoprotein that belongs to the TNF/NGF receptor family. It is encoded by TNFRSF9 (tumor necrosis factor receptor superfamily, member 9). CD137 is expressed on activated T cells, B cells, monocytes, and follicular dendritic cells. CD137 plays roles in the costimulation, differentiation and survival of T cells and B cells.

    561702 Rev. 2
    Format Details
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    APC
    Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    APC
    Red 627-640 nm
    651 nm
    660 nm
    561702 Rev.2
    Citations & References
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    Development References (5)

    1. Garni-Wagner BA, Lee ZH, Kim YJ, Wilde C, Kang CY, Kwon BS. 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans. Cell Immunol. 1996; 169(1):91-98. (Biology). View Reference
    2. Hurtado JC, Kim SH, Pollok KE, Lee ZH, Kwon BS. Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28. J Immunol. 1995; 155(7):3360-3367. (Biology). View Reference
    3. Hurtado JC, Kim YJ, Kwon BS. AAI Meeting, Abstract #841. San Francisco: 1997.
    4. Kim YJ, Kim SH, Kwon BS. AAI Meeting, Abstract #838. San Francisco: 1997.
    5. Zhou Z, Kim S, Hurtado J, et al. Characterization of human homologue of 4-1BB and its ligand. Immunol Lett. 1995; 45(1-2):67-73. (Biology). View Reference
    View All (5) View Less
    561702 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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