Alexa Fluor® 647 Mouse Anti-Human CD51
Clone NKI-M9 (RUO)
- Brand BD Pharmingen™
- Alternative Name Integrin alpha-V; alphaV; αv; ITGAV; MSK8; VNRA; VTNR
- Concentration 0.2 mg/ml
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Melanoma Cells
- Workshop No. P103
- Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The NKI-M9 monoclonal antibody specifically binds to CD51, which is also known as Integrin alpha-V (ITGAV, αv), or Vitronectin receptor subunit alpha (VNRA, VTNR). CD51 is a type I transmembrane glycoprotein that is cleaved into 125 kDa and 25 kDa subunits which are disulfide linked. CD51 can form heterodimers with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 is expressed on platelets, osteoclasts, endothelial cells, fibroblasts, macrophages, and some B cells, as well as neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs can serve as ligands for CD51. In association with various integrin β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. The CD51 adhesion molecule plays important roles in leukocyte activation and localization, bone absorption, and angiogenesis.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.