PE Mouse Anti-Human CD164
Clone N6B6 (RUO)
- Brand BD Pharmingen™
- Vol. Per Test 20 µl
- Isotype Mouse IgG2a, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Pre-B Cell Line
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The N6B6 monoclonal antibody specifically recognizes CD164. CD164 is a 80-90 kDa, mucin-like molecule present on CD34+ cells during the early stages of B cell, erythroid cell and myelomonocytic cell development. It is also expressed on epithelial cells, peripheral blood monocytes and weakly on lymphocytes. Mucin-like molecules are a new family of glycoproteins present in tissues of the hematopoietic system. They are highly glycosylated polypeptides, containing predominantly O-linked carbohydrate side chains. CD164 may reportedly play a role in hematopoiesis by facilitating the adhesion of CD34+ cells to bone marrow stroma.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.