Purified Rat Anti-Mouse CD140a
Clone APA5 (RUO)
- Brand BD Pharmingen™
- Alternative Name Pdgfra; Pdgfr2; PDGF-R alpha; Platelet derived growth factor receptor alpha
- Concentration 0.5 mg/ml
- Isotype Rat WF, also known as Wistar Furth IgG2a, κ
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
Western blot, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, ELISA, Functional assay (Reported)
- Immunogen Mouse PDGF Receptor α chain
- Entrez Gene ID 18595
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR-α). CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult. CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells. CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The APA5 antibody has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This antibody has been tested by immunofluorescent staining (≤ 1 µg/million cells) with flow cytometric analysis and immunohistochemical staining of acetone-fixed frozen sections (0.5 - 2.5 µg/ml) to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action.