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    PE Rat Anti-Mouse CD140A
    PE Rat Anti-Mouse CD140A
    Flow cytometric analysis of CD140a expression on mouse fibroblast NIH/3T3 cells. Fresh NIH/3T3 cells were stained with either PE Rat anti-Mouse CD140a antibody (Cat. No. 562776, solid line histogram) or PE Rat IgG2a, k Isotype Control (Cat. No. 555844, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
    PE Rat Anti-Mouse CD140A
    Flow cytometric analysis of CD140a expression on mouse fibroblast NIH/3T3 cells. Fresh NIH/3T3 cells were stained with either PE Rat anti-Mouse CD140a antibody (Cat. No. 562776, solid line histogram) or PE Rat IgG2a, k Isotype Control (Cat. No. 555844, dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
    Product Details
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    BD Pharmingen™
    Pdgfra; Pdgfr2; PDGF-R alpha; Platelet derived growth factor receptor alpha
    Mouse (QC Testing)
    Rat WF, also known as Wistar Furth IgG2a, κ
    Mouse PDGF Receptor α chain
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    18595
    AB_2737787
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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    562776 Rev. 1
    Antibody Details
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    APA5

    The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR-α). CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult. CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells. CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain.  Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization.  The APA5 antibody has been demonstrated to block binding of  PDGF-AA to PDGFR-a-expressing cells in vitro  and to block some PDGF-mediated developmental events in vivo.

                                  

    562776 Rev. 1
    Format Details
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    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    562776 Rev.1
    Citations & References
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    Development References (3)

    1. Fruttiger M, Calver AR, Krüger WH. PDGF mediates a neuron-astrocyte interaction in the developing retina. Neuron. 1996; 17(6):1117-1131. (Clone-specific: Blocking). View Reference
    2. Heldin CH. Structural and functional studies on platelet-derived growth factor. EMBO J. 1992; 11(12):4251-4259. (Biology). View Reference
    3. Takakura N, Yoshida H, Kunisada T, Nishikawa S, Nishikawa SI. Involvement of platelet-derived growth factor receptor-alpha in hair canal formation. J Invest Dermatol. 1996; 107(5):770-777. (Immunogen: Blocking, ELISA, Immunohistochemistry, Inhibition, Western blot). View Reference
    562776 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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