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    BV421 Mouse Anti-Mouse CD286 (TLR6)
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    This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
    BV421 Mouse Anti-Mouse CD286 (TLR6)
    Multicolor flow cytometric analysis of CD286 (TLR6) expression on mouse macrophages. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse F4/80 (Cat. No. 565410) and Alexa Fluor® 647 Rat Anti-Mouse CD11b (Cat. No. 557686) antibodies, and either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BV421 Mouse Anti-Mouse CD286 (TLR6) antibody (Cat. No. 566005; solid line histogram). The fluorescence histogram showing CD286 (TLR6) expression (or Ig Isotype control staining) was derived from CD11b-hi F4/80-hi events with the forward and side light-scatter characteristics of viable bone marrow myeloid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
    BV421 Mouse Anti-Mouse CD286 (TLR6)
    Flow cytometric analysis of CD286 (TLR6) expression on mouse RAW 264.7 cells. Cells from the RAW 264.7 (Macrophage ATCC TIB-71) cell line were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either BD Horizon BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BV421 Mouse Anti-Mouse CD286 (TLR6) antibody (Cat. No. 566005; solid line histogram). The fluorescence histogram showing CD286 (TLR6) expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
    BV421 Mouse Anti-Mouse CD286 (TLR6) BV421 Mouse Anti-Mouse CD286 (TLR6)
    Multicolor flow cytometric analysis of CD286 (TLR6) expression on mouse macrophages. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse F4/80 (Cat. No. 565410) and Alexa Fluor® 647 Rat Anti-Mouse CD11b (Cat. No. 557686) antibodies, and either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BV421 Mouse Anti-Mouse CD286 (TLR6) antibody (Cat. No. 566005; solid line histogram). The fluorescence histogram showing CD286 (TLR6) expression (or Ig Isotype control staining) was derived from CD11b-hi F4/80-hi events with the forward and side light-scatter characteristics of viable bone marrow myeloid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
    Flow cytometric analysis of CD286 (TLR6) expression on mouse RAW 264.7 cells. Cells from the RAW 264.7 (Macrophage ATCC TIB-71) cell line were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either BD Horizon BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BV421 Mouse Anti-Mouse CD286 (TLR6) antibody (Cat. No. 566005; solid line histogram). The fluorescence histogram showing CD286 (TLR6) expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
    Product Details
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    BD Horizon™
    Tlr6; Toll-like receptor 6
    Mouse (QC Testing)
    Mouse BALB/c IgG1, κ
    Mouse TLR6
    Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Reported)
    0.2 mg/ml
    21899
    AB_2739454
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    7. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    8. An isotype control should be used at the same concentration as the antibody of interest.
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    566005 Rev. 1
    Antibody Details
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    C1N2

    The C1N2 monoclonal antibody specifically binds to the Toll-like receptor 6 (TLR6), which is also known as CD286. CD286 belongs to the mammalian toll-like family of pattern recognition receptors (TLR) that participate in inducing innate immune responses to microbial pathogens. CD286 is a type I transmembrane glycoprotein comprised of an extracellular domain with a large number of leucinerich repeats (LRRs) and a cytoplasmic region with a signaling Toll/Interleukin-1 Receptor (TIR) domain. TLR6 is differentially expressed on the surface and intracellular compartments, including endolysosomes, of pre-B cells as well as immature or mature B cells. It is likewise expressed by monocytes, macrophages, or plasmacytoid, CD4+, or CD8+ dendritic cells. In association with TLR2 (CD282), CD286 recognizes microbial diacylated lipopeptides, lipoteichoic acid, and zymosan. The CD282/CD286 (TLR2/TLR60 heterodimer then transduces signaling responses leading to the production of proinflammatory cytokines by responding cells.

    566005 Rev. 1
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    566005 Rev.1
    Citations & References
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    Development References (5)

    1. Kang JY, Lee JO. Structural biology of the Toll-like receptor family.. Annu Rev Biochem. 2011; 80:917-41. (Biology). View Reference
    2. Marinho FA, de Paula RR, Mendes AC, et al. Toll-like receptor 6 senses Mycobacterium avium and is required for efficient control of mycobacterial infection.. Eur J Immunol. 2013; 43(9):2373-85. (Biology). View Reference
    3. Motoi Y, Shibata T, Takahashi K, et al. Lipopeptides are signaled by Toll-like receptor 1, 2 and 6 in endolysosomes.. Int Immunol. 2014; 26(10):563-73. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
    4. Takeuchi O, Kawai T, Sanjo H, et al. TLR6: A novel member of an expanding toll-like receptor family. Gene. 1999; 231(1-2):59-65. (Biology). View Reference
    5. de Almeida LA, Macedo GC, Marinho FA, et al. Toll-like receptor 6 plays an important role in host innate resistance to Brucella abortus infection in mice.. Infect Immun. 2013; 81(5):1654-62. (Biology). View Reference
    View All (5) View Less
    566005 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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