Purified Mouse Anti-Human CD107b
Clone H4B4 (RUO)
- Brand BD Pharmingen™
- Alternative Name LAMP-2; LAMP2; LAMPB; LGP110
- Concentration 0.5 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
- Application
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human Adult Adherent Peripheral Blood Cells
- Workshop No. V P007
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Description
The H4B4 monoclonal antibody specifically binds to CD107b. CD107b is a heavily glycosylated, ~120 kDa type I transmembrane protein that is also known as lysosomal-associated membrane protein 2 (LAMP-2/LAMP2), LAMPB, or LGP110. CD107b is expressed in the lysosomal and endosomal membranes of most cell types where it may play a role in maintaining membrane integrity. CD107b is also expressed on the surface of activated platelets and lymphocytes, including T lymphocytes, e.g., degranulating CD8+ cytotoxic T cells. CD107b serves as a ligand for selectins, including E-selectin, and mediates cellular adhesion. CD107a/ LAMP-1 and CD107b /LAMP-2 are carriers for poly-N-acetyllactosamines, and display the sialyl Le[x] antigen. CD107b is likewise expressed on some tumor cells and cell lines, including U937 and KG1a, and may facilitate tumor metastases.
Format
Suggested Companion Products
Purified Mouse IgG1, κ Isotype Control RUO
0.1 mg
Cat No: 555746
FITC Goat Anti-Mouse IgG/IgM Polyclonal RUO
0.5 mg
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BD Cytofix/Cytoperm™ Fixation/Permeablization Kit RUO
250 Tests
Cat No: 554714
Resources & Tools | ||||||
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Spectrum Viewer | Download TDS | Regulatory Document Website |
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.