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Purified Mouse Anti-Human CD107b H4B4 RUO

Purified Mouse Anti-Human CD107b 

Clone  H4B4   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name LAMP-2; LAMP2; LAMPB; LGP110
  • Concentration 0.5 mg/ml
  • Isotype Mouse IgG1, κ
  • Reactivity Human (QC Testing) 
  • Application Intracellular staining (flow cytometry) (Routinely Tested) 
  • Immunogen Human Adult Adherent Peripheral Blood Cells
  • Workshop No. V P007
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The H4B4 monoclonal antibody specifically binds to CD107b. CD107b is a heavily glycosylated, ~120 kDa type I transmembrane protein that is also known as lysosomal-associated membrane protein 2 (LAMP-2/LAMP2), LAMPB, or LGP110. CD107b is expressed in the lysosomal and endosomal membranes of most cell types where it may play a role in maintaining membrane integrity. CD107b is also expressed on the surface of activated platelets and lymphocytes, including T lymphocytes, e.g., degranulating CD8+ cytotoxic T cells. CD107b serves as a ligand for selectins, including E-selectin, and mediates cellular adhesion. CD107a/ LAMP-1 and CD107b /LAMP-2 are carriers for poly-N-acetyllactosamines, and display the sialyl Le[x] antigen. CD107b is likewise expressed on some tumor cells and cell lines, including U937 and KG1a, and may facilitate tumor metastases.

Format
  • Format Purified

Other Formats →

Suggested Companion Products

Purified Mouse IgG1, κ Isotype Control RUO
0.1 mg Cat No: 555746

FITC Goat Anti-Mouse IgG/IgM Polyclonal RUO
0.5 mg Cat No: 555988

BD Cytofix/Cytoperm™ Fixation/Permeablization Kit RUO
250 Tests Cat No: 554714

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


  1. Chen JW, Cha Y, Yuksel KU, Gracy RW, August JT. Isolation and sequencing of a cDNA clone encoding lysosomal membrane glycoprotein mouse LAMP-1. Sequence similarity to proteins bearing onco-differentiation antigens. J Biol Chem. 1988; 263(18):8754-8758. View reference

  2. Febbraio M, Silverstein RL. Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein. J Biol Chem. 1990; 265(30):18531-18537. View reference

  3. Sawada R, Lowe JB, Fukuda M. E-selectin-dependent adhesion efficiency of colonic carcinoma cells is increased by genetic manipulation of their cell surface lysosomal membrane glycoprotein-1 expression levels. J Biol Chem. 1993; 268(17):12675-12681. View reference

  4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995; .

555803 Rev. 1
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