Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Middle East / Africa (English)
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
    Alert Icon
    Alexa Fluor® 488 Mouse Anti-Human BMI-1
    Alexa Fluor® 488 Mouse Anti-Human BMI-1
    Image analysis of BMI-1 expressed in human osteosarcoma cell line. U-2 OS cells (ATCC; HTB-96™) were fixed with BD Cytofix™ Buffer (Cat. No. 554655), permeabilized with 0.1% Triton™ X-100 (Sigma, Cat. No. X-100), and stained with Alexa Fluor® 488 Mouse Anti-Human-BMI-1 antibody (5 μg/ml; pseudo-colored green). Counter-staining was with DAPI (pseudo-colored blue). The image was captured using a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.
    Alexa Fluor® 488 Mouse Anti-Human BMI-1
    Flow cytometric analysis of BMI-1 expression in a human osteosarcoma cell line. U-2 OS cells  (ATCC; HTB-96™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (dashed line histogram, Cat. No. 557782) or Alexa Fluor® 488 Mouse anti-Human BMI-1 antibody (solid line histogram, Cat. No. 562636) at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD™ LSR II Flow Cytometry System.
    Alexa Fluor® 488 Mouse Anti-Human BMI-1 Alexa Fluor® 488 Mouse Anti-Human BMI-1
    Image analysis of BMI-1 expressed in human osteosarcoma cell line. U-2 OS cells (ATCC; HTB-96™) were fixed with BD Cytofix™ Buffer (Cat. No. 554655), permeabilized with 0.1% Triton™ X-100 (Sigma, Cat. No. X-100), and stained with Alexa Fluor® 488 Mouse Anti-Human-BMI-1 antibody (5 μg/ml; pseudo-colored green). Counter-staining was with DAPI (pseudo-colored blue). The image was captured using a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.
    Flow cytometric analysis of BMI-1 expression in a human osteosarcoma cell line. U-2 OS cells  (ATCC; HTB-96™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (dashed line histogram, Cat. No. 557782) or Alexa Fluor® 488 Mouse anti-Human BMI-1 antibody (solid line histogram, Cat. No. 562636) at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD™ LSR II Flow Cytometry System.
    Product Details
    Down Arrow Up Arrow


    BD Pharmingen™
    BMI1; PCGF4; polycomb group ring finger 4; RING finger protein 51; RNF51
    Human (QC Testing)
    Mouse BALB/c IgG1, κ
    Human BMI-1 Recombinant Protein
    Intracellular staining (flow cytometry) (Routinely Tested)
    5 µl
    648
    AB_2737692
    Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
    Show More blue down arrow Show Less blue up arrow

    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. An isotype control should be used at the same concentration as the antibody of interest.
    4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
    6. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
    7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    8. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    10. All other brands are trademarks of their respective owners.
    11. Triton is a trademark of the Dow Chemical Company.
    Show More blue down arrow Show Less blue up arrow
    562636 Rev. 1
    Antibody Details
    Down Arrow Up Arrow
    P51-311

    The P51-311 monoclonal antibody binds to human BMI-1 (B lymphoma Mo-MLV insertion region 1 homolog). BMI1 is a c-myc cooperating oncogene that encodes an ~45 kDa protein that is a member of the Polycomb Group (PcG) of proteins. PcG proteins are essential for the maintenance, but not initiation, of the transcriptionally repressed state of certain developmental genes. PcG proteins are a structurally diverse group of proteins with conserved functions from fly to human cells. PcG proteins form complexes and regulate the expression of genes involved in cell cycle, DNA repair and differentiation that are crucial for maintaining the self renewal of normal and cancer stem cells. Specifically, BMI-1 is a core component of PRC1 (polycomb repressive complex 1). BMI-1, via the up-regulation of hTERT and independent of c-myc, can immortalize mammary epithelial cells. BMI-1 has also been shown to repress the INK4A locus that controls the tumor suppressors p16 and p19ARF (mouse homologue of p14ARF) in mouse models. BMI-1 plays a role in maintaining the self-renewal capacities of stem cells including hematopoietic, intestinal, retinal and neural stem cells.  During antibody development, the purified P51-311 monoclonal antibody was found to detect BMI-1 by Western blot analysis of cellular lysates and by indirect immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells.

    562636 Rev. 1
    Format Details
    Down Arrow Up Arrow
    Alexa Fluor™ 488
    Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    Alexa Fluor™ 488
    Blue 488 nm
    494 nm
    517 nm
    562636 Rev.1
    Citations & References
    Down Arrow Up Arrow

    Development References (4)

    1. Dimri GP, Martinez JL, Jacobs JJ. The Bmi-1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Cancer Res. 2002; 62(16):4736-4745. (Biology). View Reference
    2. Molofsky AV, Pardal R, Iwashita T, Park IK, Clarke MF, Morrison SJ. Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation. Nature. 2003; 6961(962):967. (Biology). View Reference
    3. Park IK, Qian D, Kiel M, et al. Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells. Nature. 2003; 423(6937):302-305. (Biology). View Reference
    4. Sangiorgi, E., Capecchi, M. R. Bmi1 is expressed in vivo in intestinal stem cells. Nat Genet. 2008; 40(7):915-920. (Biology). View Reference
    View All (4) View Less
    562636 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

    Website Feedback