Purified Hamster Anti-Mouse CD80
Clone 16-10A1 (RUO)
- Brand BD Pharmingen™
- Alternative Name Cd80; B7; B7-1; Cd28l; Ly-53; MIC17; TSA1
- Concentration 0.5 mg/ml
- Isotype Armenian Hamster IgG2, κ
- Reactivity Mouse (QC Testing) Dog (Reported)
Flow cytometry (Routinely Tested)
In vivo exacerbation, Blocking, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
- Immunogen Mouse CD80 (B7) Transfected Cell Line
- Entrez Gene ID 12519
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 16-10A1 monoclonal antibody specifically recognizes CD80 (B7-1). This member of the Ig superfamily, like CD86 (B7-2), can bind to either CD28 or CD152 (CTLA-4) and provide either costimulatory or coinhibitory signals to T cells, respectively. CD80 is constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages as well as by activated B cells and T cells. The 16-10A1 antibody blocks binding of CTLA-4 Ig to CD80 as well as T-cell activation by Con A-elicited peritoneal exudate cells or CD80-transfected cell lines. However, the 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells. The 16-10A1 antibody may reportedly block the binding of another CD80-specific antibody, clone 1G10. In addition, the 16-10A1 antibody may crossreact with an activation antigen expressed on IFN-γ-activated alveolar macrophages of the dog.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Precautions for flow cytometry: For flow cytometry of leukocytes, it is recommended that Mouse BD Fc Block™ (Cat. No. 553141) be used. When using Mouse BD Fc Block™ (clone 2.4G2), it is important that the second-step anti-hamster IgG antibody does not cross-react with the 2.4G2 mAb (rat IgG2b, κ); we have found that the anti-hamster IgG cocktail (Cat. No. 554010) to be effective. Since this antigen is expressed at low density, it may be desirable to amplify staining by using a biotinylated second-step reagent followed by a "bright" third-step reagent, such as streptavidin-PE (Cat. No. 554061).
Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.