Biotin Rat Anti-Mouse CD123

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BD Pharmingen™ Biotin Rat Anti-Mouse CD123

Name: Biotin Rat Anti-Mouse CD123
Brand: BD Pharmingen™
SKU: 555070

Clone 5B11

(RUO)

Flow cytometric analysis of CD123 (IL-3 Receptor α chain) on mouse bone marrow cells. C57BL/B6 bone marrow leukocytes were stained with either Biotin Rat Anti-Mouse CD123 (Cat. No. 555070, Left Panel) or Biotin Rat IgG2a, κ isotype control (Cat. No. 553928, Right Panel)) followed by PE Streptavidin (Cat. No. 554061) and FITC Rat Anti-CD11b (Cat. No. 557396). Two-color dot plots showing CD11b and CD123 (or Ig isotype control) expression were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACSCanto™ II system.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Il3ra; IL-3RA; IL-3R-alpha; IL-3 Receptor alpha; IL-3 Receptor α; SUT-1

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2a, κ

Immunogen: Mouse IL-3R transfected CTLL cells

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_395683

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Biotin Rat IgG2a κ Isotype Control RUO

Size 0.25 mg Cat No. 553928

PE Streptavidin RUO

Size 0.5 mg Cat No. 554061

555070 Rev. 2

Antibody Details

5B11

The 5B11 monoclonal antibody specifically recognizes mouse CD123, the α chain subunit (IL-3Rα) of the mouse IL-3 receptor. The IL-3Rα chain is a 60-70 kD transmembrane glycoprotein that binds IL-3 with low affinity but cannot transduce signals by itself. The mouse IL-3Rα chain can complex with either of two homologous β chain subunits (βc and βIL-3) to form high-affinity heterodimeric IL-3 receptors. The βc chain is the common β chain subunit that can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors. The βIL-3 subunit can form high affinity IL-3 receptor complexes with only the IL-3Rα subunit. The βIL-3 subunit clone is specific for mouse IL-3 and binds with low affinity. The immunogen used to generate the 5B11 hybridoma was CTLL cells transfected with the mouse IL-3R alpha chain. The 5B11 antibody does not block the binding of IL-3 protein to the high affinity IL-3 receptor heterodimer.

555070 Rev. 2

Format Details

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

Format: Biotin

555070 Rev.2

Citations & References

Development References (3)

Hara T, Miyajima A. Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3). EMBO J. 1992; 11(5):1875-1884. (Biology). View Reference
Ichihara M, Hara T, Takagi M, Cho LC, Gorman DM, Miyajima A. Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene. EMBO J. 1995; 14(5):939-950. (Biology). View Reference
Mueller DL, Chen ZM, Schwartz RH, Gorman DM, Kennedy MK. Subset of CD4+ T cell clones expressing IL-3 receptor alpha-chains uses IL-3 as a cofactor in autocrine growth. J Immunol. 1994; 153(7):3014-3027. (Biology). View Reference

555070 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.