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FITC Mouse Anti-Rat Ig, κ Light Chain MRK-1 RUO

FITC Mouse Anti-Rat Ig, κ Light Chain 

Clone  MRK-1   (RUO)

  • Brand BD Pharmingen™
  • Concentration 0.5 mg/ml
  • Isotype Mouse IgG1
  • Reactivity Rat (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Pooled rat Ig
  • Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The MRK-1 monoclonal antibody reacts specifically with rat Igs bearing κ light chains. It does not react with rat immunoglobulin λ light chains or heavy chains. The MRK-1 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

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Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


FITC-conjugated MRK-1 mAb may be used as a primary or secondary reagent in immunofluorescent staining.

553872 Rev. 10
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