HRP Anti-Human IgG
Clone G18-145 (RUO)
- Brand BD Pharmingen™
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
ELISA (Routinely Tested)
- Storage Buffer Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
- Regulatory Status RUO
Regulatory Status Legend
IgG is an important component of the humoral immune response, helping to control infection. IgG is produced by plasma B-cells and may be found in extracellular fluids, such as blood, lymph, peritoneal, and cerebrospinal fluids. IgG monomers consist of two light and two heavy chains containing two antigen binding sites. There are four IgG subclasses found in human, mouse and rat species, which include IgG1, IgG2, IgG3 and IgG4. The G18-145 monoclonal antibody specifically binds to the heavy chain of human immunoglobulin G subclasses: IgG1, IgG2, IgG3 and IgG4. The G18-145 antibody has been reported not to react with the heavy chains of other human immunoglobulin isotypes.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- ProClin is a trademark of Rohm and Haas Company.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Investigators are encouraged to titrate, with a suggested starting dilution of 1:1000. For sandwich ELISA of human IgG4, HRP-conjugated G18-145 is optimal as detecting mAb with purified JDC-14 (Cat. No. 555878) or G17-4 (Cat. No. 555881) as capture mAb.
Warning: HRP Mouse Anti-Human IgG contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).
May cause an allergic skin reaction.
Wear protective gloves / eye protection.
Wear protective clothing.
Avoid breathing mist/vapours/spray.
If skin irritation or rash occurs: Get medical advice/attention.
IF ON SKIN: Wash with plenty of water.
Dispose of contents/container in accordance with local/regional/national/international regulations.