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    Purified Mouse Anti-Human Cyclin A
    Purified Mouse Anti-Human Cyclin A
    Western blot analysis of cyclin A.  K-562 cell lysates (Human bone marrow myelogenous leukemia; ATCC CCL-243) were probed with the mouse anti-human cyclin A antibody at concentration of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3). Cyclin A may be identified as a band migrating between 54-60 kDa.
    Purified Mouse Anti-Human Cyclin A
    Western blot analysis of cyclin A.  K-562 cell lysates (Human bone marrow myelogenous leukemia; ATCC CCL-243) were probed with the mouse anti-human cyclin A antibody at concentration of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3). Cyclin A may be identified as a band migrating between 54-60 kDa.
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    BD Pharmingen™
    Human (QC Testing)
    Mouse IgE
    Human Cyclin A Recombinant Protein
    Western blot (Routinely Tested), Flow cytometry (Tested During Development), Immunoprecipitation, In vitro kinase assay (Reported)
    54-60 kDa
    0.5 mg/ml
    AB_395285
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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    Recommended Assay Procedures

    Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    554174 Rev. 9
    Antibody Details
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    BF683

    Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved  proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. Cyclin A is involved in both S-phase and G2/M transitions of the cell cycle through its association with cdk2 and cdk1, respectively. Cyclin A may also form a complex with the adenovirus oncoprotein E1A which has DNA binding activity. Human cyclin A has been reported to migrate between 54-60 kDa by SDS-PAGE and clone BF683 reportedly does not cross-react with mouse, rat or mink cyclin A.

    554174 Rev. 9
    Format Details
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    Purified
    Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
    Purified
    554174 Rev.9
    Citations & References
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    Development References (6)

    1. Cao L, Faha B, Dembski M, Tsai LH, Harlow E, Dyson N. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F. Nature. 1992; 355(6356):176-179. (Clone-specific: Immunoprecipitation, Western blot). View Reference
    2. Faha B, Ewen ME, Tsai LH, Livingston DM, Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. Science. 1992; 255(5040):87-90. (Clone-specific: Immunoprecipitation, In vitro kinase assay, Western blot). View Reference
    3. Faha B, Harlow E, Lees E. The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2. J Virol. 1993; 67(5):2456-2465. (Clone-specific: Immunoprecipitation, In vitro kinase assay). View Reference
    4. Gong J, Bhatia U, Traganos F, Darzynkiewicz Z. Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry. Leukemia. 1995; 9(5):893-899. (Clone-specific: Flow cytometry). View Reference
    5. Gong J, Traganos F, Darzynkiewicz Z. Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1. Exp Cell Res. 1995; 220(1):226-231. (Clone-specific: Flow cytometry, Western blot). View Reference
    6. Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology). View Reference
    View All (6) View Less
    554174 Rev. 9

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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