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Purified Mouse Anti- Bcl-x

BD Pharmingen™ Purified Mouse Anti- Bcl-x

Clone 2H12

(RUO)
Purified Mouse Anti- Bcl-x
Western blot analysis of Bcl-x expression on Jurkat cell lysate. Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with Purified Mouse Anti- Bcl-x antibody (Cat. No. 551022/551020) at concentrations of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3), and visualized with HRP Goat Anti-Mouse Ig secondary antibody (Cat. No. 554002).
Western blot analysis of Bcl-x expression on Jurkat cell lysate. Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with Purified Mouse Anti- Bcl-x antibody (Cat. No. 551022/551020) at concentrations of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3), and visualized with HRP Goat Anti-Mouse Ig secondary antibody (Cat. No. 554002).
Product Details
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BD Pharmingen™
Western blot (Routinely Tested)
RUO
AB_394007


Description

Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. Some Bcl-2 family members like Bcl-2, block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-x is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-xL (long) is a 241 amino acid protein (25-29 kDa) that is 47% homologous to Bcl-2 on the amino acid level. Bcl-xS (short) is a 178 amino acid protein (~ 19.5 kDa) lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. Bcl-xL blocks cell death, whereas Bcl-xS inhibits Bcl-2 and promotes cell death.

The 2H12 antibody has been reported to recognize human, mouse and rat Bcl-xL (long) protein. An N-terminal peptide (amino acids 3-14) common to human and mouse Bcl-xL and Bcl-xS (short) proteins was used as the immunogen. Thus the antibody is also predicted to recognize the Bcl-xS protein as the sequence used for the immunogen is common to both Bcl-xL and Bcl-xS proteins. Reports from development, however, have indicated that Bcl-xS has not been observable.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

In addition to Jurkat cell lysates,  MOLT-4 (Human T-lymphoblasts; ATCC CRL-1582), K-562 (Human bone marrow myelogenous leukemia; ATCC CCL-243), Hut-78 (Human T lymphoma; ATCC TIB-161), human peripheral blood mononuclear cells (PBMC), rat thymocytes and mouse thymocytes have also been reported to be useful as positive controls.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
551022 Rev. 3
Components
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Description Quantity/Size Part Number EntrezGene ID
Purified Mouse Anti- Bcl-x 50 µg (3 ea) 51-6646GR N/A
Jurkat Cell Lysate 50 µg (1 ea) 51-16526N N/A
551022 Rev. 3
Citations & References
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Development References (4)

  1. Hsu YT, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A. 1997; 94(8):3668-3672. (Biology: Western blot). View Reference
  2. Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997; 272(21):13829-13834. (Immunogen: Western blot). View Reference
  3. Krajewski S, Krajewska M, Shabaik A, et al. Immunohistochemical analysis of in vivo patterns of Bcl-X expression. Cancer Res. 1994; 54(21):5501-5507. (Biology). View Reference
  4. Reed JC, Miyashita T, Krajewski S, et al. Bcl-2 family proteins and the regulation of programmed cell death in leukemia and lymphoma. Cancer Treat Res. 1996; 84:31-72. (Biology). View Reference
551022 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.