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Lysing Solution 10X Concentrate CE/IVD

Lysing Solution 10X Concentrate 

  • Brand BD FACS™
  • Application Flow cytometry (Routinely Tested) 
  • Storage Buffer Buffered solution with <15% formaldehyde and <50% diethylene glycol.
  • Regulatory Status CE/IVD
Product Details Recommended Assay References

Description

BD FACS™ lysing solution is intended for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

BD FACS lysing solution is appropriate for use with reagents such as BD Tritest™ or BD Simultest™ reagents and a suitably equipped flow cytometer. It may be used in both lyse/wash and lyse/no-wash procedures.

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Preparation and Storage

BD FACS lysing solution (10X) is stable until the expiration date shown on the bottle label when stored as directed. Do not use this reagent if discoloration occurs or a precipitate forms.


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  2. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline—Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2008. C28-A3.

  3. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard—Sixth Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2007. H3-A6.

  4. Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline—Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.

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  10. Landay AL, Muirhead KA. Procedural guidelines for performing immunophenotyping by flow cytometry. Clin Immunol Immunopath. 1989;52:48-60.

  11. Nicholson JK, Rao PE, Calvelli T, et al. Artifactual staining of monoclonal antibodies in two-color combinations is due to an immunoglobulin in the serum and plasma. Cytometry. 1994;18:140-146.

  12. Prince HE, Hirji K, Waldbeser LS, Plaeger-Marshall S, Kleinman S, Lanier LL. Influence of racial background on the distribution of T-cell subsets and Leu 11-positive lymphocytes in healthy blood donors. Diagn Immunol. 1985;3(1):33-37.

  13. Renzi P, Ginns LC. Analysis of T cell subsets in normal adults: comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry. J Immunol Methods. 1987;98:53-56.

  14. Romeu MA, Mestre M, González L, et al. Lymphocyte immunophenotyping by flow cytometry in normal adults: comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation. J Immunol Methods. 1992;154:7-10.

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