Skip to main content Skip to navigation
Purified Mouse Anti-PBK
Purified Mouse Anti-PBK
Western blot analysis of PBK on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-PBK antibody.
Western blot analysis of PBK on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-PBK antibody.
Product Details
Down Arrow Up Arrow

BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG2b
Human PBK aa. 191-322
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
41 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612171 Rev. 1
Antibody Details
Down Arrow Up Arrow

Mitogen activated protein (MAP) kinase signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAP kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases, MKK3 and MKK6. PBK is a PDZ-binding Ser-Thr kinase related to the MKK3/6 MAPKK family. PBK was also identified as T-LAK cell- originated protein kinase (TOPK) and spermatogenesis-related protein kinase (SPK). PBK contains the conserved dual specficity active site sequence (D-X-K-X-X-N) at amino acids 174 to 179, and a C-terminal ETDV motif that binds PDZ domains. The mRNA expression of PBK is reportedly highest in placenta, and lower in heart and pancreas. In mitotic cells, PBK is phosphorylated and enzymatically active, and in vitro, PBK is phosphorylated by Cdc2/cyclin B. PBK can bind to the PDZ domain-containing protein, Dlg, and can phosphorylate p38 MAPK. Thus, PBK  is a MAPKK family member that may be important for regulation of PDZ domain proteins during mitosis.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612171 Rev. 1
Format Details
Down Arrow Up Arrow
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
612171 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Abe Y, Matsumoto S, Kito K, Ueda N. Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells. J Biol Chem. 2000; 275(28):21525-21531. (Biology). View Reference
  2. Gaudet S, Branton D, Lue RA. Characterization of PDZ-binding kinase, a mitotic kinase. Proc Natl Acad Sci U S A. 2000; 97(10):5167-5172. (Biology). View Reference
  3. Zhao S, Dai J, Zhao W. PDZ-binding kinase participates in spermatogenesis. Int J Biochem Cell Biol. 2001; 33(6):631-636. (Biology). View Reference
612171 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.