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      Purified Mouse Anti-MAP1B
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      This SKU will be discontinuing June 2023. For additional support, contact your local applications specialist. Contact Us #
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      BD Transduction Laboratories™
      Mouse (QC Testing), Human, Rat (Tested in Development)
      Mouse IgG2a
      Mouse MAP1B aa. 1745-1858
      Western blot (Routinely Tested)
      320-340 kDa
      250 µg/ml
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      612679 Rev. 1
      Antibody Details
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      6/MAP1B

      Microtubule-associated proteins (MAPs) play a crucial role in the development and structure of nerve cells. These proteins are important for the assembly and stability of microtubles during neurite outgrowth and for the morphology of neuronal processes, such as dendrites. MAP1B encodes for a single precursor protein that is cleaved to produce a single heavy chain of 280 kDa and a light chain of 32 kDa which become noncovalently associated. Expression levels of MAP1B are reportedly highest in the brain and are modulated during development as reported with gene silencing experiments that implicate MAP1B's role during neuronal differentiation. In addition, MAP1B can be selectively phosphorylated depending on the cell type, subcellular localization and developmental phase. In neurons, MAP1B is expressed as a glycoprotein at the plasma membrane, where it can interact with proteins participating in axonal guiding. MAP1B has been reported to be observable to migrate in a range between 320-340 kDa.

      This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      612679 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      612679 Rev.1
      Citations & References
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      Development References (2)

      1. Ma D, Himes BT, Shea TB, Fischer I. Axonal transport of microtubule-associated protein 1B (MAP1B) in the sciatic nerve of adult rat: distinct transport rates of different isoforms. J Neurosci. 2000; 20(6):2112-2120. (Biology). View Reference
      2. Togel M, Wiche G, Propst F. Novel features of the light chain of microtubule-associated protein MAP1B: microtubule stabilization, self interaction, actin filament binding, and regulation by the heavy chain. J Cell Biol. 1998; 143(3):695-707. (Biology). View Reference
      612679 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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