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Purified Mouse Anti-Adaptin ε
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Adaptin ε aa. 685-793
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
127 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612018 Rev. 1
Antibody Details
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32/Adaptin ε

Sorting of integral membrane proteins is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large adaptin proteins (γ, α, σ, ε and β1, β2, β3, β4 respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, µ4 respectively) and one small chain (σ1, σ2, σ3, σ4 respectively). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. AP-4 is associated with non-clathrin coated vesicles in the region of the TGN. This localization is disrupted by brefeldin A, indicating that AP-4 membrane attachment is regulated by small GTPases. The µ4 subunit of the AP-4 complex interacts with tyrosine-based signals on LAMP-2 during targeting to the endosomal-lysosomal system. Thus, AP-4 is a less abundant AP complex that may be important for vesicle trafficking from the Golgi to the endosomal-lysosomal system.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (3)

  1. Aguilar RC, Boehm M, Gorshkova I. Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4. J Biol Chem. 2001; 276(16):13145-13152. (Biology). View Reference
  2. Dell'Angelica EC, Mullins C, Bonifacino JS. AP-4, a novel protein complex related to clathrin adaptors. J Biol Chem. 1999; 274(11):7278-7285. (Biology). View Reference
  3. Hirst J, Bright NA, Rous B, Robinson MS. Characterization of a fourth adaptor-related protein complex. Mol Biol Cell. 1999; 10(8):2787-2802. (Biology). View Reference
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.