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Purified Mouse Anti-CD95
Purified Mouse Anti-CD95

Western blot analysis of CD95 on a Daudi cell lysate (Human B lymphoblast; ATCC CCL-213). Lane 1:5,000, lane 2: 1:10,000, lane 3: 1:20,000 in dilution of thePurified Mouse anti-CD95 antibody.

Purified Mouse Anti-CD95

Immunohistochemical staining of a formalin-fixed rabbit liver section.

Western blot analysis of CD95 on a Daudi cell lysate (Human B lymphoblast; ATCC CCL-213). Lane 1:5,000, lane 2: 1:10,000, lane 3: 1:20,000 in dilution of thePurified Mouse anti-CD95 antibody.

Immunohistochemical staining of a formalin-fixed rabbit liver section.

Product Details
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BD Transduction Laboratories™
Fas, APO-1
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a, κ
Human Fas aa. 1-163
Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development), Immunofluorescence, Immunoprecipitation (Not Recommended)
45 kDa
250 µg/ml
AB_2100251
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

For Western Blot and immunohistochemistry resources, please reference http://www.bdbiosciences.com/resources/cellbiology/index.jsp

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610197 Rev. 1
Antibody Details
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13/Fas

CD95 is a member of a family of cell surface receptors that includes tumor necrosis factor receptor (TNF-R), nerve growth factor receptor (NGF-R), CD40, CD27, CD30, and 4-1BB. Both murine and human Fas genes have been cloned and reportedly share 60% similarity in their amino acid sequences. CD95 (Fas) is a cell surface apoptosis-signaling molecule that is widely expressed in sites such as thymus, liver, heart, and ovary. Abnormalities in the Fas gene correlate with autoimmune features in mice and with unusually high levels of lymphocyte apoptosis in HIV-infected humans. Genetic studies have localized the Fas gene near the lpr (lymphoproliferation disease) locus on mouse chromosome 19 and further characterization reportedly has revealed that lpr is a mutation affecting the function of the Fas gene.

610197 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610197 Rev.1
Citations & References
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Development References (5)

  1. Arnold R, Seifert M, Asadullah K, Volk HD. Crosstalk between keratinocytes and T lymphocytes via Fas/Fas ligand interaction: modulation by cytokines.. J Immunol. 1999; 162(12):7140-7147. (Biology: Flow cytometry). View Reference
  2. Itoh N, Yonehara S, Ishii A, et al. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell. 1991; 66(2):233-243. (Biology). View Reference
  3. MacLaren A, Clark W, Gillespie DA. v-Jun sensitizes cells to apoptosis by a mechanism involving mitochondrial cytochrome C release.. Oncogene. 2000 ; 19(51):5906-5918. (Biology: Western blot). View Reference
  4. Rosen K, Coll ML, Li A, Filmus J. Transforming growth factor-alpha prevents detachment-induced inhibition of c-Src kinase activity, Bcl-XL down-regulation, and apoptosis of intestinal epithelial cells.. 2001; 276(40):37273-37279. (Clone-specific: Western blot). View Reference
  5. Zhuang S, Demirs JT, Kochevar IE. Protein kinase C inhibits singlet oxygen-induced apoptosis by decreasing caspase-8 activation.. Oncogene. 2001; 20(46):6764-6776. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610197 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.