BD™ Cytometric Bead Array (CBA) Phospho PLC-γ (Y783) Flex Set
(RUO)
Description
The BD™ CBA Phospho PLC-γ (Y783) Flex Set is a bead-based immunoassay capable of measuring human, mouse or rat Phospholipase C-γ (PLC-γ) that has been tyrosine-phosphorylated at Tyr-783 in denatured cell lysate samples. Human reactivity was determined by testing cell lysates with the BD CBA Phospho PLC-γ (Y783) Flex Set. Reactivity with mouse and rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho PLC-γ (Y783) Flex Set. There are several publications evaluating the biology and function of PLC-γ. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
Preparation And Storage
The Phospho PLC-γ (Y783) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
Recommended Assay Procedures
The BD CBA Phospho PLC-γ (Y783) Flex Set must be used in conjunction with a BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005, 100 tests, or 560006, 500 tests) and a flow cytometer. Detailed instructions on the use of this product can be found in the manual for the BD CBA Cell Signaling Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Phospho PLC-γ (Y783) Flex Set will be 1,000 Units/mL. An example standard curve is shown in Figure 1.
The BD CBA Phospho PLC-γ (Y783) Flex Set should not be used in the same assay well with any non-BD CBA Cell Signaling Flex Set reagents (such as BD CBA Human or Mouse Soluble Protein Flex Sets) nor with any BD CBA Total Protein Cell Signaling Flex Set reagents. For an updated assay compatibility chart for the BD CBA Cell Signaling Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.
Performance
Limit of Detection: The theoretical limit of detection is 1.77 Units/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 Units/mL, n=30) + 2 standard deviations.
Product Notices
- ProClin is a trademark of Rohm and Haas Company.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Warning: CBA lyophilized standard contains 32.07% sodium dodecyl sulfate (w/w) and 0.01% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May be harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause an allergic skin reaction.Precautionary statements: Wear protective gloves/eye protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Dispose of contents/container in accordance with local/regional/national/international regulations.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Phospho PLC-γ (Y783) Capture Bead B7 | 100 Tests (1 ea) | 51-9005182 | N/A |
| Phospho PLC-γ (Y783) PE Detection Reagent | 100 Tests (1 ea) | 51-9003885 | N/A |
| Phospho PLC-γ (Y783) Standard | 100 Tests (1 ea) | 51-9005180 | N/A |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.