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      RY610 Mouse Anti-Human CD309 (VEGFR2)
      Product Details
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      BD OptiBuild™
      CD309; FLK-1; FLK1; KDR; VEGFR-2; VEGFR2
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human VEGFR-2
      Flow cytometry (Qualified)
      0.2 mg/ml
      3791
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. An isotype control should be used at the same concentration as the antibody of interest.
      10. CF™ is a trademark of Biotium, Inc.
      11. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      759685 Rev. 1
      Antibody Details
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      BB13-1044

      The BB13-1044 monoclonal antibody specifically recognizes Vascular endothelial growth factor receptor 2 (VEGFR-2) which is also known as CD309 and Fetal liver kinase 1 (FLK-1). This protein receptor tyrosine kinase is an ~230 kDa single pass type I transmembrane glycoprotein that is encoded by KDR (Kinase insert domain receptor). It is variably expressed by mesodermal and endothelial cells in embryonic tissues and by vascular endothelial cells, megakaryocytes, platelets, retinal progenitors, and smooth muscle cells in normal adult or tumor tissues. The N-terminal extracellular region of CD309 (VEGFR-2) consists of seven IgC2-like domains which are followed by a single transmembrane region and a C-terminal intracellular tyrosine kinase. The first three extracellular domains mediate ligand binding whereas the remaining four are involved in receptor dimerization. CD309 (VEGFR-2) functions as a receptor for several Vascular endothelial growth factor (VEGF) isoforms. Following receptor dimerization upon ligand binding, CD309 (VEGFR-2) autophosphorylates and stimulates the proliferation and migration of endothelial and other cell types.

      759685 Rev. 1
      Format Details
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      RY610
      The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.
      altImg
      RY610
      Yellow-Green 561 nm
      557 nm
      610 nm
      759685 Rev.1
      Citations & References
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      View product citations for antibody "759685" on CiteAb

      Development References (3)

      1. Ferrara N, Houck K, Jakeman L, Leung DW. Molecular and biological properties of the vascular endothelial growth factor family of proteins. Endocr Res. 1992; 13(1):18-32. (Biology). View Reference
      2. Yang L, Soonpaa MH, Adler ED, et al. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature. 2008; 453(7194):524-528. (Biology). View Reference
      3. Ziegler BL, Valtieri M, Porada GA, et al. KDR receptor: a key marker defining hematopoietic stem cells. Science. 1999; 285:1553-1558. (Biology). View Reference
      759685 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.