Skip to main content Skip to navigation
RB744 Mouse Anti-Human TCR Cβ2
RB744 Mouse Anti-Human TCR Cβ2
Multiparameter flow cytometric analysis of TCR Cβ2 expression on Human peripheral blood lymphocytes.  Human whole blood was stained with PE Mouse Anti-Human Cβ1 TCR antibody (Cat. No. 565776) and with either BD Horizon™ RB744 Mouse IgG1, κ Isotype Control (Cat. No. 570519; Left Plot) or BD Horizon™ RB744 Mouse Anti-Human TCR Cβ2 antibody (Cat. No. 571685/571758; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of TCR Cβ2 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of TCR Cβ2 expression on Human peripheral blood lymphocytes.  Human whole blood was stained with PE Mouse Anti-Human Cβ1 TCR antibody (Cat. No. 565776) and with either BD Horizon™ RB744 Mouse IgG1, κ Isotype Control (Cat. No. 570519; Left Plot) or BD Horizon™ RB744 Mouse Anti-Human TCR Cβ2 antibody (Cat. No. 571685/571758; Right Plot) at 0.25 µg/test. Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of TCR Cβ2 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
Down Arrow Up Arrow


BD Horizon™
T cell receptor beta constant 2; TCR Cβ2; TCRBC2; TRBC2
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.2 mg/ml
28638
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  9. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
  10. Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
  11. For U.S. patents that may apply, see bd.com/patents.
571758 Rev. 1
Antibody Details
Down Arrow Up Arrow
SAM.2.rMAb

The SAM.2.rMAb is a recombinant monoclonal antibody that recognizes TCR Cβ2 expressed by a large proportion of CD4+ and CD8+ T cells. Thymocytes and mature peripheral T cells predominantly express a heterodimeric T cell receptor (TCR αβ) for antigen which is comprised of disulfide-liked transmembrane α and β chain subunits. The constant region of the TCR α subunit is encoded by TRAC, whereas the TCR β subunit is encoded by either of two highly homologous constant region genes, TCRB1 for TCR Cβ1 or TCRB2 for TCR Cβ2. The JOVI.1 antibody alternatively recognizes TCR Cβ1 expressed by the other TCR αβ+ T cells. These antibodies are effectively used together in multicolor staining and flow cytometric analyses to identify and characterize the natures of either TCR Cβ1+ or TCR Cβ2+ T cells in heterogeneous cell populations.

Note - Some human CD3- and TCR-specific antibodies might not be compatible to co-stain human T cells with SAM2.rMab. To confirm compatibility and immunofluorescent staining protocols, please visit: https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/discover-learn/thought-leadership/events/POSTER-AAI%20202024-S-Riguad.pdf

571758 Rev. 1
Format Details
Down Arrow Up Arrow
RB744
The BD Horizon RealBlue™ 744 (RB744) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 746-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB744 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), we recommend using an optical filter centered near 750-nm (e.g., a 750/60-nm bandpass filter).
altImg
RB744
Blue 488 nm
498 nm
746 nm
571758 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "571758" on CiteAb

Development References (9)

  1. Berg H, Otteson GE, Corley H, et al. Flow cytometric evaluation of TRBC1 expression in tissue specimens and body fluids is a novel and specific method for assessment of T-cell clonality and diagnosis of T-cell neoplasms.. Cytometry B Clin Cytom. 2021; 100(3):361-369. (Biology). View Reference
  2. Ferrari M, Baldan V, Ghongane P, et al. Targeting TRBC1 and 2 for the treatment of T cell lymphomas. Abstract. Cancer Res. 2020; 80:2183. (Biology).
  3. Ferrari M, Righi M, Baldan V, et al. Structure-guided engineering of immunotherapies targeting TRBC1 and TRBC2 in T cell malignancies.. Nat Commun. 2024; 15(1):1583. (Clone-specific: Flow cytometry). View Reference
  4. Horna P, Shi M, Olteanu H, Johansson U. Emerging Role of T-cell Receptor Constant β Chain-1 (TRBC1) Expression in the Flow Cytometric Diagnosis of T-cell Malignancies.. Int J Mol Sci. 2021; 22(4):1817. (Biology). View Reference
  5. Horna P, Weybright MJ, Ferrari M, et al. Dual T-cell constant β chain (TRBC)1 and TRBC2 staining for the identification of T-cell neoplasms by flow cytometry.. Blood Cancer J. 2024; 14(1):34. (Clone-specific: Flow cytometry). View Reference
  6. Maciocia PM, Wawrzyniecka PA, Philip B, et al. Targeting the T cell receptor β-chain constant region for immunotherapy of T cell malignancies.. Nat Med. 2017; 23(12):1416-1423. (Biology). View Reference
  7. Morath A, Schamel WW. αβ and γδ T cell receptors: Similar but different.. J Leukoc Biol. 2020; 107(6):1045-1055. (Biology). View Reference
  8. Muñoz-García N, Lima M, Villamor N, et al. Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation.. Cancers (Basel). 2021; 13(17):4379. (Biology). View Reference
  9. Tesio M. Subtle Differences to Make the Difference.. Hemasphere. 2018; 2(2):e38. (Biology). View Reference
View All (9) View Less
571758 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.