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      RB613 Mouse Anti-Human IgA1
      Product Details
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      BD OptiBuild™
      IGHA1; Immunoglobulin alpha 1
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human IgA1 Myeloma Protein
      Flow cytometry (Qualified)
      0.2 mg/ml
      3493
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. An isotype control should be used at the same concentration as the antibody of interest.
      10. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      11. CF™ is a trademark of Biotium, Inc.
      12. Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
      13. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      772220 Rev. 1
      Antibody Details
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      B3506B4

      The B3506B4 monoclonal antibody specifically recognizes the Fc region of the human Immunoglobulin A (IgA) subclass known as IgA1. IgA1 is comprised of two identical heavy chains encoded by IGHA1, and two light chains, either Igκ or Igλ, that are linked together by disulfide bonds. The B3506B4 antibody does not crossreact with other human immunoglobulin heavy chain (IgH) subclasses, including the IgA2 subclass. IgA1 is expressed by plasmablasts, plasma cells, and memory B cells as well as by some myeloma or plasmacytoma cells. IgA1 is expressed in either a transmembrane form that serves as an antigen-specific B cell surface receptor or in a soluble secreted form. Monomeric and dimeric forms of IgA1 are found in the serum although the monomeric form predominates. Multimeric forms of IgA1 connected by a J-chain and secretory component are also found in bodily secretions. Secretory IgA is transported across epithelial cells and secreted into the lumen of mucosal tissues including the gastrointestinal and respiratory tracts and is found in human breast milk. IgA1 functions in the agglutination of microbes and can block their adherence to or infection of human cells. It can also bind to CD89 (FcαRI) expressed on neutrophils, monocytes, macrophages, and eosinophils to trigger a variety of immune responses including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and the release of inflammatory cytokines and mediators such as in the response to microbes.

      772220 Rev. 1
      Format Details
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      RB613
      The BD Horizon RealBlue™ 613 (RB613) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 492-nm and an emission maximum (Em Max) at 613-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB613 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with reduced excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB613 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PE-CF594. RB613 is on average brighter than PE-CF594 off the blue laser.
      altImg
      RB613
      Blue 488 nm
      492 nm
      613 nm
      772220 Rev.1
      Citations & References
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      View product citations for antibody "772220" on CiteAb

      Development References (9)

      1. Avery DT, Bryant VL, Ma CS, de Waal Malefyt R, Tangye SG. IL-21-induced isotype switching to IgG and IgA by human naive B cells is differentially regulated by IL-4.. J Immunol. 2008; 181(3):1767-79. (Clone-specific: Flow cytometry). View Reference
      2. Blanco E, Perez-Andres M, Sanoja-Flores L, et al. Selection and validation of antibody clones against IgG and IgA subclasses in switched memory B-cells and plasma cells.. J Immunol Methods. 2019; 475:112372. (Clone-specific: Flow cytometry). View Reference
      3. Blanco E, Pérez-Andrés M, Arriba-Méndez S, et al. Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood.. J Allergy Clin Immunol. 2018; 141(6):2208-2219.e16. (Clone-specific: Flow cytometry). View Reference
      4. Delacroix DL, Van Snick J, Vaerman JP, Conley ME, Mascart-Lemone F, Bernier GM. Monoclonal antibodies against isotypic and isoallotypic determinants of human IgA1 and IgA2: fine specificities and binding properties.. Mol Immunol. 1986; 23(4):367-75. (Immunogen: Radioimmunoassay). View Reference
      5. Fayette J, Dubois B, Vandenabeele S, et al. Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2.. J Exp Med. 1997; 185(11):1909-18. (Biology). View Reference
      6. He B, Xu W, Santini PA, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL.. Immunity. 2007; 26(6):812-26. (Clone-specific: Flow cytometry). View Reference
      7. Sibille Y, Chatelain B, Staquet P, Merrill WW, Delacroix DL, Vaerman JP. Surface IgA and Fc-alpha receptors on human alveolar macrophages from normal subjects and from patients with sarcoidosis.. Am Rev Respir Dis. 1989; 139(3):740-7. (Clone-specific: Blocking). View Reference
      8. Tangye SG, Ferguson A, Avery DT, Ma CS, Hodgkin PD. Isotype switching by human B cells is division-associated and regulated by cytokines.. J Immunol. 2002; 169(8):4298-306. (Biology). View Reference
      9. Zan H, Cerutti A, Dramitinos P, Schaffer A, Casali P. CD40 engagement triggers switching to IgA1 and IgA2 in human B cells through induction of endogenous TGF-beta: evidence for TGF-beta but not IL-10-dependent direct S mu-->S alpha and sequential S mu-->S gamma, S gamma-->S alpha DNA recombination.. J Immunol. 1998; 161(10):5217-25. (Biology). View Reference
      View All (9) View Less
      772220 Rev. 1

       

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      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.