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Purified Mouse Anti-Human Prominin-1 (CD133)
Purified Mouse Anti-Human Prominin-1 (CD133)
Analyses of Prominin-1 (CD133) expression      Left Panel.  Flow cytometric analysis of Prominin-1 (CD133) expression on human WERI-Rb-1 cells. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 553454; dashed line histogram) or Purified Mouse Anti-Human Prominin-1 (CD133) antibody (Cat. No. 566794; solid line histogram) at 0.5 μg/test. The cells were washed and stained with FITC Goat Anti-Mouse Ig secondary antibody (Cat. No. 554001). The fluorescence histogram showing Prominin-1 (CD133) expression (or Ig Isotype control staining) was derived from gated events with forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.      Right Panel. Immunohistochemical staining of Prominin-1 (CD133) in Human Colon Cancer. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed, paraffin-embedded sections of human colon cancer were stained with either Purified Mouse IgG2a κ Isotype Control (Cat. No. 550339; Left Image) or Purified Mouse Anti-Human Prominin-1 (CD133) antibody overnight (Cat. No. 566794; Right Image). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. As shown in the Right Figure, the Human Prominin-1 (CD133) antibody primarily stained the surface colon cancer cells. Original magnification: 20×.
Analyses of Prominin-1 (CD133) expression      Left Panel.  Flow cytometric analysis of Prominin-1 (CD133) expression on human WERI-Rb-1 cells. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 553454; dashed line histogram) or Purified Mouse Anti-Human Prominin-1 (CD133) antibody (Cat. No. 566794; solid line histogram) at 0.5 μg/test. The cells were washed and stained with FITC Goat Anti-Mouse Ig secondary antibody (Cat. No. 554001). The fluorescence histogram showing Prominin-1 (CD133) expression (or Ig Isotype control staining) was derived from gated events with forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.      Right Panel. Immunohistochemical staining of Prominin-1 (CD133) in Human Colon Cancer. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed, paraffin-embedded sections of human colon cancer were stained with either Purified Mouse IgG2a κ Isotype Control (Cat. No. 550339; Left Image) or Purified Mouse Anti-Human Prominin-1 (CD133) antibody overnight (Cat. No. 566794; Right Image). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. As shown in the Right Figure, the Human Prominin-1 (CD133) antibody primarily stained the surface colon cancer cells. Original magnification: 20×.
Product Details
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BD Pharmingen™
PROM1; PROML1; hProminin; AC133; MCDR2; STGD4; CORD12; MSTP061; RP41
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human Prominin-1 Transfected Cell Line
Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-paraffin, Western blot (Tested During Development)
0.5 mg/ml
AB_2869872
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566794 Rev. 1
Antibody Details
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CMab-43

The CMab-43 monoclonal antibody specifically recognizes Prominin-1, which is also known as Prominin (mouse)-like 1 (PROML1 or Prominin-like protein 1), hProminin, Hematopoietic stem cell antigen, Macular dystrophy retinal 2 (MCDR2), Stargardt disease 4 autosomal dominant (STGD4), AC133 antigen, or CD133. Prominin-1 (CD133) is a ~120 kDa five-transmembrane glycoprotein that is encoded by PROM1 (Prominin 1) which belongs to the Prominin gene family. This single-chain, pentaspan transmembrane glycoprotein is comprised of an extracellular N-terminus with two short intracellular sequences and two long extracellular loops followed by an intracellular C-terminus. Prominin-1 (CD133) is expressed on hematopoietic stem cells and progenitor cells found in human bone marrow, cord blood and peripheral blood. It is also expressed on neural stem cells, other tissue-specific stem cells, endothelial progenitor cells, epithelial cells during development, and retinal cells. Prominin-1 (CD133) is likewise detected on some cancer cells from solid tumors, leukemias, cell lines, and can serve as a marker for cancer stem cells. Mutations in PROM1 have reportedly been associated with some retinal diseases.

                

566794 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
566794 Rev.1
Citations & References
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Development References (4)

  1. Itai S, Fujii Y, Nakamura T, et al. Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry. Monoclon Antib Immunodiagn Immunother. 2017; 36(5):231-235. (Immunogen: Flow cytometry, Immunohistochemistry, Western blot). View Reference
  2. Maw MA, Corbeil D, Koch J, et al. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration.. Hum Mol Genet. 2000; 9(1):27-34. (Biology). View Reference
  3. Miraglia SJ, Buck D. CD133 (AC133). In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:870-872.
  4. Permanyer J, Navarro R, Friedman J, et al. Autosomal recessive retinitis pigmentosa with early macular affectation caused by premature truncation in PROM1.. Invest Ophthalmol Vis Sci. 2010; 51(5):2656-63. (Biology). View Reference
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566794 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.