BD Pharmingen™ Purified Mouse Anti-Human CD34
Clone QBEND/10.rMAb (also known as QB-END-10, QBEND10, QBEND/10, QBEnd10) (RUO)
Flow cytometric analysis of CD34 expression on KG1a cell line. KG1a (ATCC® CCL-246.1™) cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat No. 556648; dashed line histogram) or Purified Mouse Anti-Human CD34 antibody (Cat No. 568773; solid line histogram), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) [Cat No. 550589] secondary antibody at 0.25 µg/test. The fluorescence histogram showing the expression of CD34 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CD34 expression on KG1a cell line. KG1a (ATCC® CCL-246.1™) cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat No. 556648; dashed line histogram) or Purified Mouse Anti-Human CD34 antibody (Cat No. 568773; solid line histogram), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) [Cat No. 550589] secondary antibody at 0.25 µg/test. The fluorescence histogram showing the expression of CD34 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Preparation And Storage
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
The QBEND/10.rMab recombinant monoclonal antibody specifically recognizes human CD34, a 105-120 kDa single-chain type I transmembrane glycoprotein, also known as CD34 antigen. CD34 is expressed on immature hematopoietic progenitor cells and all hematopoietic colony-forming cells in the bone marrow and blood, including vascular endothelium cells and some tissue fibroblasts. Normal peripheral blood lymphocytes, monocytes, granulocytes, and platelets do not express CD34. CD34 density is highest on early hematopoietic progenitor cells and decreases as cells mature. The antigen is absent on fully differentiated hematopoietic cells. The cytoplasmic region of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting CD34 may play a role in signal transduction. CD34 has also been named as a possible adhesion molecule by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. The QBEND/10.rMAb antibody recognizes an epitope on CD34 distinct from those recognized by clones My10, 563, 581, and 8G12.
Development References (6)
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Orfao A, Matarraz S, Pérez-Andrés M, et al. Immunophenotypic dissection of normal hematopoiesis.. J Immunol Methods. 2019; 475:112684. (Biology: Flow cytometry). View Reference
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Radtke S, Görgens A, Kordelas L, et al. CD133 allows elaborated discrimination and quantification of haematopoietic progenitor subsets in human haematopoietic stem cell transplants.. Br J Haematol. 2015; 169(6):868-78. (Biology: Flow cytometry). View Reference
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Ramani P, Bradley NJ, Fletcher CD. QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections.. Histopathology. 1990; 17(3):237-42. (Immunogen: Immunohistochemistry). View Reference
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Sankey EA, More L, Dhillon AP. QBEnd/10: a new immunostain for the routine diagnosis of Kaposi's sarcoma.. J Pathol. 1990; 161(3):267-71. (Clone-specific: Immunohistochemistry). View Reference
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Sutherland DR, Keating A. The CD34 antigen: structure, biology, and potential clinical applications.. J Hematother. 1992; 1(2):115-29. (Clone-specific: Cell separation, Flow cytometry). View Reference
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Sutherland DR, Stewart AK, Keating A. CD34 antigen: molecular features and potential clinical applications.. Stem Cells. 1993; 11 Suppl 3:50-7. (Clone-specific: Cell separation, Flow cytometry). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.