Purified Mouse Anti-Human CD150

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BD Pharmingen™ Purified Mouse Anti-Human CD150

Name: Purified Mouse Anti-Human CD150
Brand: BD Pharmingen™
SKU: 559591

Clone A12

(RUO)

Profile of peripheral blood lymphocytes analyzed by flow cytometry.

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Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Activated Human Peripheral Blood Mononuclear Cells

Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)

Concentration: 0.5 mg/ml

RRID: AB_397285

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

Purified Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 555746

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

559591 Rev. 1

Antibody Details

A12

The A12 monoclonal antibody specifically binds to CD150, a single chain type I transmembrane glycoprotein of approximately 75-95 kDa. CD150 is encoded by the SLAMF1 gene and is also known as a signaling lymphocyte activation molecule (SLAM), or IPO-3. It is expressed on T cells, B cells, thymocytes, germinal center and dendritic cells, but not on monocytes. Expression of CD150 is upregulated on T, B and dendritic cells after activation via T-cell and B-cell costimulatory molecules. It has been reported that CD150 plays a role in signal transduction.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

559591 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

559591 Rev.1

Citations & References

View product citations for antibody "559591" on CiteAb

Development References (3)

Cocks BG, Chang CC, Carballido JM, Yssel H, de Vries JE, Aversa G. A novel receptor involved in T-cell activation. Nature. 1995; 376(6537):260-263. (Biology). View Reference
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Sidorenko SP, Clark EA. Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes. J Immunol. 1993; 151(9):4614-4624. (Biology). View Reference

559591 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.