Product Details
BD Pharmingen™
CC chemokine receptor 3; CC-CKR3; Ccr3
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Mouse CCR3 Transfected Cells
Flow cytometry, Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Rat IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554685
APC Rat Anti-Mouse Ly-6G and Ly-6C RUO
Size
0.1 mg
Cat No.
553129
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
DAPI Solution RUO
Size
1 mg
Cat No.
564907
572244 Rev. 1
Antibody Details
C3Mab-4.rMAb
The C3Mab-4.rMAb is a recombinant monoclonal antibody derived from C3Mab-4 hybridoma cells that specifically recognizes CD193, which is also known as, C-C chemokine receptor type 3 (CCR3). This seven transmembrane G protein-coupled chemokine receptor is encoded by Ccr3 (C-C motif chemokine receptor 3) and can transduce extracellular signals to intracellular signaling molecule pathways when bound by certain chemokines. CD193 (CCR3) serves as a chemokine receptor for CCL11 (Eotaxin), CCL24 (Eotaxin-2), and CCL26 (Eotaxin-3). It also binds to several other chemokines including CCL3 (MIP-1a), CCL5 (RANTES), CCL7 (MCP-3), CCL8 (MCP-2), CCL14 (MCP-4), and CCL15 (HCC-2). CD193 (CCR3) is reportedly expressed on eosinophils, mast cells, mononuclear phagocytes, Th2-like cells, hematopoietic progenitor cells, platelets, and epithelial cells. When chemokine ligand bound, this receptor plays an important role in the recruitment of eosinophils and other leukocytes to the skin and the lungs. This can lead to the development of airway hyperresponsiveness associated with rhinitis and asthma.
572244 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
572244 Rev.1
Citations & References
View product citations for antibody "572244" on CiteAb
Development References (5)
-
Asano T, Nanamiya R, Takei J, et al. Development of Anti-Mouse CC Chemokine Receptor 3 Monoclonal Antibodies for Flow Cytometry.. Monoclon Antib Immunodiagn Immunother. 2021; 40(3):107-112. (Biology). View Reference
-
Ma W, Bryce PJ, Humbles AA. CCR3 is essential for skin eosinophilia and airway hyperresponsiveness in a murine model of allergic skin inflammation. J Clin Invest. 2002; 109(5):621-628. (Biology). View Reference
-
Tateyama N, Asano T, Suzuki H, et al. Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies Using Flow Cytometry.. Antibodies (Basel). 2022; 11(4):75. (Immunogen: Flow cytometry). View Reference
-
Tateyama N, Asano T, Tanaka T, et al. Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies (C3Mab-6 and C3Mab-7).. Monoclon Antib Immunodiagn Immunother. 2023; 42(2):68-72. (Biology). View Reference
-
Teixeira MM, Wells TN, Lukacs NW. Chemokine-induced eosinophil recruitment. Evidence of a role for endogenous eotaxin in an in vivo allergy model in mouse skin. J Clin Invest. 1997; 100(7):1657-1666. (Biology). View Reference
572244 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.