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PE Rat Anti-Mouse CD131
PE Rat Anti-Mouse CD131

Flow cytometric analysis of βIL-3 and CD131 expression on Mac-1+ bone marrow cells. BALB/c bone marrow cells (BMC) were stained (20 minutes at 4°C) with PE Rat Anti-Mouse CD131 antibody (1 µg mAb/10^6 cells; Cat. No. 559920) and FITC Rat Anti-Mouse CD11b (Cat. No. 553310). The two-color fluorescence dot plot shows the correlated expression patterns of CD11b versus βIL-3 and CD131 with the forward and side light-scatter characteristics of viable bone marrow cells.

Flow cytometric analysis of βIL-3 and CD131 expression on Mac-1+ bone marrow cells. BALB/c bone marrow cells (BMC) were stained (20 minutes at 4°C) with PE Rat Anti-Mouse CD131 antibody (1 µg mAb/10^6 cells; Cat. No. 559920) and FITC Rat Anti-Mouse CD11b (Cat. No. 553310). The two-color fluorescence dot plot shows the correlated expression patterns of CD11b versus βIL-3 and CD131 with the forward and side light-scatter characteristics of viable bone marrow cells.

Product Details
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BD Pharmingen™
CD131, βc, Bc, AIC2B, Il3rb1, Csf2rb1; βIL3, BIL3, AIC2A, Il3rb2, Csf2rb2
Mouse (QC Testing)
Rat IgG1, κ
Bone Marrow-Derived C4-77 Pro-T Lymphocyte Clone
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
AB_397374
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559920 Rev. 2
Antibody Details
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JORO50

The JORO50 monoclonal antibody specifically recognizes the extracellular regions of the mouse CD131/βc (Bc, AIC2B, Il3rb1, Csf2rb1) and βIL3 (BIL3, AIC2A, Il3rb2, Csf2rb2) cytokine receptor subunits. A variety of mouse cell types, including multipotential hematopoietic stem cells, mast cells, megakaryocytes, eosinophils, erythroblasts, pre-B cells, and osteoclasts, express βIL-3 and βc subunits. Either βIL-3 or βc can combine with the IL-3Rα chain to form two distinct, functional (i.e., signaling), high affinity receptors for mouse IL-3. The βIL-3 subunit by itself can bind mouse IL-3 with low affinity whereas the βc subunit can not. βc (but not βIL-3) can combine with the IL-5Rα (CD125) and GM-CSFRα (CD116) subunits to form high affinity, signaling receptors for mouse IL-5 or GM-CSF, respectively. The immunogen used to generate the JORO50 hybridoma was the bone marrow-derived, C4-77 pro-T lymphocyte clone.

559920 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
559920 Rev.2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.