Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      PE Mouse Anti-Mouse CD198 (CCR8)
      PE Mouse Anti-Mouse CD198 (CCR8)
      Multicolor flow cytometric analysis of CD198 (CCR8) expression on Mouse thymocytes. C57BL/6 Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with APC Rat Anti-Mouse CD4 (Cat No. 553051) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Mouse CD198 (CCR8) antibody (Cat. No. 571903/571904; Right Plot) at 1 μg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD198 (CCR8) [or Ig Isotype control staining] versus CD4 was derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      PE Mouse Anti-Mouse CD198 (CCR8)
      Multicolor flow cytometric analysis of CD198 (CCR8) expression on Mouse thymocytes. C57BL/6 Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with APC Rat Anti-Mouse CD4 (Cat No. 553051) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Mouse CD198 (CCR8) antibody (Cat. No. 571903/571904; Right Plot) at 1 μg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD198 (CCR8) [or Ig Isotype control staining] versus CD4 was derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      C-C chemokine receptor type 8; Ccr8; mCCR8
      Mouse (QC Testing)
      Mouse IgG1, κ
      Mouse CCR8 Transfected Cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12776
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. For U.S. patents that may apply, see bd.com/patents.
      Show More blue down arrow Show Less blue up arrow
      571903 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      5C12.rMAb

      The 5C12.RMAB is a recombinant monoclonal antibody that was derived from the 5C12 clone that specifically recognizes mouse CD198 which is also known as C-C chemokine receptor type 8 (CCR8). Clone 5C12 was originally generated from a Ccr8-knockout mouse with a C57BL/6 background that was immunized with mouse Ccr8-transfected cells. CD198 (CCR8) is an ~41 kDa seven transmembrane G protein-coupled chemokine receptor that is encoded by Ccr8 (C-C motif chemokine receptor 8). It is variably expressed on subsets of thymocytes, Th2-like cells, Tregs, NKT cells, CD8+ T cells, as well as monocytes, macrophages, and dendritic cells (DC). CCR8 binds to and mediates cellular chemotactic responses to its ligand CCL1 which is also known as I-309 or TCA-3.

      571903 Rev. 1
      Format Details
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 561 nm
      496 nm, 566 nm
      576 nm
      571903 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "571903" on CiteAb

      Development References (3)

      1. Coghill JM, Fowler KA, West ML, et al. CC chemokine receptor 8 potentiates donor Treg survival and is critical for the prevention of murine graft-versus-host disease.. Blood. 2013; 122(5):825-36. (Biology). View Reference
      2. Goya I1, Villares R, Zaballos A, et al. Absence of CCR8 does not impair the response to ovalbumin-induced allergic airway disease.. J Immunol. 2003; 170(4):2138-2146. (Biology). View Reference
      3. Kremer L1, Carramolino L, Goya I, et al. The transient expression of C-C chemokine receptor 8 in thymus identifies a thymocyte subset committed to become CD4+ single-positive T cells.. J Immunol. 2001; 166(1):218-225. (Biology). View Reference
      571903 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.